ArticlePlant physiology2013
Divergent DNA-binding specificities of a group of ETHYLENE RESPONSE FACTOR transcription factors involved in plant defense.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 77 citations in OpenAlex.
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- Involvement of Phytohormone Responses of Rubber Leaves after Pestalotiopsis microspora Infection Revealed with Transcriptome Analysis.The plant pathology journal · 2026Article
- The Diversity of the DNA-Binding Landscape in the DREB/ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous Genome.Plant biotechnology journal · 2026Article
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Evolutionary trajectory of transcription factors and selection of targets for metabolic engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- The R2R3 MYB Ruby1 is activated by two cold responsive ethylene response factors, via the retrotransposon in its promoter, to positively regulate anthocyanin biosynthesis in citrus.The Plant journal : for cell and molecular biology · 2024Article
- ACRE, a class of AP2/ERF transcription factors, activates the expression of sweet potato ß-amylase and sporamin genes through the sugar-responsible element CMSRE-1.Plant molecular biology · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Plant disease resistance outputs regulated by AP2/ERF transcription factor family.Stress biology · 2024Review
- Regulatory mechanisms of the LBD40 transcription factor inPlant direct · 2023Article
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- Comparative and expression analyses of AP2/ERF genes reveal copy number expansion and potential functions of ERF genes in Solanaceae.BMC plant biology · 2023Article
- Natural and induced variations in transcriptional regulator genes result in low-nicotine phenotypes in tobacco.The Plant journal : for cell and molecular biology · 2022Article
- Genetic divergence in transcriptional regulators of defense metabolism: insight into plant domestication and improvement.Plant molecular biology · 2022Review
- The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants.Protoplasma · 2022Article
- APETALA2/ethylene responsive factor in fruit ripening: Roles, interactions and expression regulation.Frontiers in plant science · 2022Review
- Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor inInternational journal of molecular sciences · 2021Article
- Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice inFrontiers in plant science · 2021Article
- Degradome, small RNAs and transcriptome sequencing of a high-nicotine cultivated tobacco uncovers miRNA's function in nicotine biosynthesis.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factors (TFs) recognize target DNA sequences with distinct DNA-binding domains (DBDs). The DBD of Arabidopsis (Arabidopsis thaliana) ETHYLENE RESPONSE FACTOR1 (AtERF1) uses three consecutive β-strands to recognize a GCC-containing sequence, but tobacco (Nicotiana tabacum) ERF189 and periwinkle (Catharanthus roseus) Octadecanoid-derivative Responsive Catharanthus AP2-domain protein3 (ORCA3) of the same TF subgroup appear to target similar but divergent DNA sequences. Here, we examined how DNA-binding specificities of these TFs have diverged in each plant lineage to regulate distinct defense metabolisms. Extensive mutational analyses of these DBDs suggest that two modes of protein-DNA interactions independently contribute to binding specificity and affinity. Substitution of a conserved arginine to lysine in the first β-strand of ERF189 relaxes its interaction with the second GC pair of the GCC DNA sequence. By contrast, an increased number of basic amino acids in the first two β-strands of ORCA3 allows this TF to recognize more than one GCC-related target, presumably via increased electrostatic interactions with the negatively charged phosphate backbone of DNA. Divergent DNA-binding specificities of the ERFs may have arisen through mutational changes of these amino acid residues.
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