ArticleThe Journal of biological chemistry2013
Campylobacter jejuni lipooligosaccharide sialylation, phosphorylation, and amide/ester linkage modifications fine-tune human Toll-like receptor 4 activation.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Genomic variability of lipooligosaccharide biosynthesis locus and sequence type amongMicrobiology spectrum · 2025Article
- Therapeutic and protective approaches to combatFrontiers in pharmacology · 2025Review
- Consortium ofAntibiotics (Basel, Switzerland) · 2024Article
- Effect of the polysaccharide capsule and its heptose on the resistance of Campylobacter jejuni to innate immune defenses.MicrobiologyOpen · 2024Article
- Article
- Comparison of lipooligosaccharides from human challenge strains ofFrontiers in microbiology · 2023Article
- Lipid A heterogeneity and its role in the host interactions with pathogenic and commensal bacteria.microLife · 2022Review
- The ProbioticAnimals : an open access journal from MDPI · 2021Article
- Murine Models for the Investigation of Colonization Resistance and Innate Immune Responses in Campylobacter Jejuni Infections.Current topics in microbiology and immunology · 2021Article
- Predominant phosphorylation patterns inJournal of lipid research · 2020Article
- Novel ClinicalMicroorganisms · 2020Review
- An Updated Classification System and Review of the Lipooligosaccharide Biosynthesis Gene Locus inFrontiers in microbiology · 2020Review
- Human Immunity AgainstImmune network · 2019Review
- Top-Down Characterization of Lipooligosaccharides from Antibiotic-Resistant Bacteria.Analytical chemistry · 2019Article
- Comparative genomics and genome biology ofEmerging microbes & infections · 2019Article
- NovelJournal of lipid research · 2018Article
- Hypoacylated LPS from Foodborne PathogenFrontiers in cellular and infection microbiology · 2018Article
- Whole Genome Sequencing and Multiplex qPCR Methods to IdentifyFrontiers in microbiology · 2018Article
- Biosynthesis and structure-activity relationships of the lipid a family of glycolipids.Current opinion in chemical biology · 2017Review
- Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Campylobacter jejuni is a leading cause of acute gastroenteritis. C. jejuni lipooligosaccharide (LOS) is a potent activator of Toll-like receptor (TLR) 4-mediated innate immunity. Structural variations of the LOS have been previously reported in the oligosaccharide (OS) moiety, the disaccharide lipid A (LA) backbone, and the phosphorylation of the LA. Here, we studied LOS structural variation between C. jejuni strains associated with different ecological sources and analyzed their ability to activate TLR4 function. MALDI-TOF MS was performed to characterize structural variation in both the OS and LA among 15 different C. jejuni isolates. Cytokine induction in THP-1 cells and primary monocytes was correlated with LOS structural variation in each strain. Additionally, structural variation was correlated with the source of each strain. OS sialylation, increasing abundance of LA d-glucosamine versus 2,3-diamino-2,3-dideoxy-d-glucose, and phosphorylation status all correlated with TLR4 activation as measured in THP-1 cells and monocytes. Importantly, LOS-induced inflammatory responses were similar to those elicited by live bacteria, highlighting the prominent contribution of the LOS component in driving host immunity. OS sialylation status but not LA structure showed significant association with strains clustering with livestock sources. Our study highlights how variations in three structural components of C. jejuni LOS alter TLR4 activation and consequent monocyte activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.