ArticleThe Journal of biological chemistry2013
Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Functional properties and skin care effects of sodium trehalose sulfate.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Trial
- Kallikrein related peptidases 7 and 10 and their substrate desmoglein 3 are upregulated in early stage pancreatic cancerous lesions.Scientific reports · 2026Article
- Proton-activated chloride channel PACC1 as acid sensor in epidermal desquamation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Secukinumab as a Novel Treatment for Chronic Netherton Syndrome in a Young Adult.The American journal of case reports · 2025Article
- PRSS3/mesotrypsin as a putative regulator of the biophysical characteristics of epidermal keratinocytes in superficial layers.Scientific reports · 2024Article
- Ovol1/2 loss-induced epidermal defects elicit skin immune activation and alter global metabolism.EMBO reports · 2023Article
- Excess filaggrin in keratinocytes is removed by extracellular vesicles to prevent premature death and this mechanism can be hijacked by Staphylococcus aureus in a TLR2-dependent fashion.Journal of extracellular vesicles · 2023Article
- Extracellular epimorphin impairs expression and processing of profilaggrin in HaCaT keratinocytes.Cytotechnology · 2023Article
- Article
- Skin barrier dysfunction and filaggrin.Archives of pharmacal research · 2021Review
- On the surface: Skin microbial exposure contributes to allergic disease.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2020Review
- Kallikreins: Essential epidermal messengers for regulation of the skin microenvironment during homeostasis, repair and disease.Matrix biology plus · 2020Article
- Review
- Direct Quantification of Natural Moisturizing Factors in Stratum Corneum using Direct Analysis in Real Time Mass Spectrometry with Inkjet-Printing Technique.Scientific reports · 2019Article
- Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation.Scientific reports · 2019Article
- Microbiome of the Skin and Gut in Atopic Dermatitis (AD): Understanding the Pathophysiology and Finding Novel Management Strategies.Journal of clinical medicine · 2019Review
- Lowering relative humidity level increases epidermal protein deimination and drives human filaggrin breakdown.Journal of dermatological science · 2017Article
- Staphylococcus aureus Induces Increased Serine Protease Activity in Keratinocytes.The Journal of investigative dermatology · 2017Article
- Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice.PloS one · 2017Article
- KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filaggrin protein is synthesized in the stratum granulosum of the skin and contributes to the formation of the human skin barrier. Profilaggrin is cleaved by proteolytic enzymes and converted to functional filaggrin, but its processing mechanism remains not fully elucidated. Kallikrein-related peptidase 5 (KLK5) is a major serine protease found in the skin, which is secreted from lamellar granules following its expression in the stratum granulosum and activated in the extracellular space of the stratum corneum. Here, we searched for profilaggrin-processing protease(s) by partial purification of epidermal extracts and found KLK5 as a possible candidate. We used high performance liquid chromatography coupled with electrospray tandem mass spectrometry to show that KLK5 cleaves profilaggrin. Furthermore, based on a proximity ligation assay, immunohistochemistry, and immunoelectron microscopy analysis, we reveal that KLK5 and profilaggrin co-localize in the stratum granulosum in human epidermis. KLK5 knockdown in normal cultured human epidermal keratinocytes resulted in higher levels of profilaggrin, indicating that KLK5 potentially functions in profilaggrin cleavage.
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