ArticleAvicenna journal of medical biotechnology2013
RORC2 gene silencing in human Th17 cells by siRNA: design and evaluation of highly efficient siRNA.
Article in Avicenna journal of medical biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Homologs of Human Dengue-Resistance Genes,Insects · 2019Article
- Evaluation of the T helper 17 cell specific genes and the innate lymphoid cells counts in the peripheral blood of patients with the common variable immunodeficiency.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2014Article
- Genetic defects and the role of helper T-cells in the pathogenesis of common variable immunodeficiency.Advanced biomedical research · 2014Review
- Positive and Negative Regulation of Th17 Cell Differentiation: Evaluating The Impact of RORC2.Cell journal · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. RORC2 is the key transcription factor orchestrating Th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. The aim of this study was to design efficient siRNAs specific for RORC2 and to evaluate different criteria affecting their functionality.
methodsThree siRNA duplexes specific for RORC2 mRNA were designed. Th17 cells were produced from IL-6 and IL-1 treated cord blood CD4(+) T cells. The T cells were transfected with three different designed siRNAs against RORC2 and the expression of RORC2 gene was measured using quantitative real time PCR.
resultsDifferent levels of RORC2 down regulation were observed in the presence of each of the designed siRNAs. Efficient siRNA with 91.1% silencing activity met the majority of the established bioinformatics criteria while the one with 46.6% silencing activity had more deviations from these criteria.
conclusionThe more bioinformatics criteria are considered, the more functionality were observed for silencing RORC2. However, the importance of the type of criteria per se should not be neglected. Although all recommended criteria are important for designing siRNA but their value is not the same.
Indexed as
Identifiers
23626872PMC3572701W2115257841What OpenQuestion holds
Registered trials
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