ArticlePloS one2013
Induction of viable but nonculturable Escherichia coli O157:H7 by high pressure CO2 and its characteristics.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 121 citations in OpenAlex.
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- Salinity and Phenol-Induced VBNC State of Quorum-Quenching Bacterium and Its Resuscitation Strategies for Biofilm Control in Industrial Wastewater Treatment.Environmental microbiology · 2026Article
- How Salmonella Works Under Osmotic and Desiccation Stresses.Comprehensive reviews in food science and food safety · 2026Review
- The Effect of Non-Thermal Processing on the Fate of Pathogenic Bacteria and Hidden Hazardous Risks.Foods (Basel, Switzerland) · 2025Review
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Intracellular ATP concentration is a key regulator of bacterial cell fate.Journal of bacteriology · 2024Article
- Preliminary Study of the Characterization of the Viable but Noncultivable State ofMicroorganisms · 2024Article
- Resuscitation of viable but nonculturable bacteria promoted by ATP-mediated NADJournal of advanced research · 2024Article
- Understanding the transition to viable but non-culturable state in Escherichia coli W3110: a comprehensive analysis of potential spectrochemical biomarkers.World journal of microbiology & biotechnology · 2024Article
- The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria.Microorganisms · 2023Review
- Preliminary Study on Rapid and Simultaneous Detection of ViableMolecules (Basel, Switzerland) · 2023Article
- Susceptibility of Campylobacter jejuni to Stressors in Agrifood Systems and Induction of a Viable-but-Nonculturable State.Applied and environmental microbiology · 2023Article
- Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.Foods (Basel, Switzerland) · 2022Review
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Exploring potential bacterial populations for enhanced anthraquinone dyes biodegradation: a critical review.Biotechnology letters · 2022Review
- A Phage Receptor-Binding Protein as a Promising Tool for the Detection ofFrontiers in microbiology · 2022Article
- Identification of Quinone Degradation as a Triggering Event for Intense Pulsed Light-Elicited Metabolic Changes inMetabolites · 2021Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Stress Resistance and Pathogenicity of Nonthermal-Plasma-Induced Viable-but-Nonculturable Staphylococcus aureus through Energy Suppression, Oxidative Stress Defense, and Immune-Escape Mechanisms.Applied and environmental microbiology · 2021Article
- Role of amoebae for survival and recovery of 'non-culturable' Helicobacter pylori cells in aquatic environments.FEMS microbiology ecology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The viable but nonculturable (VBNC) state is a survival strategy adopted by many pathogens when exposed to harsh environmental stresses. In this study, we investigated for the first time that whether high pressure CO2 (HPCD), one of the nonthermal pasteurization techniques, can induce Escherichia coli O157:H7 into the VBNC state. By measuring plate counts, viable cell counts and total cell counts, E. coli O157:H7 in 0.85% NaCl solution (pH 7.0) was able to enter the VBNC state by HPCD treatment at 5 MPa and four temperatures (25°C, 31°C, 34°C and 37°C). Meanwhile, with the improvement of treatment temperature, the time required for E. coli O157:H7 to enter VBNC state would shorten. Enzymatic activities in these VBNC cells were lower than those in the exponential-phase cells by using API ZYM kit, which were also reduced with increasing the treatment temperature, but the mechanical resistance of the VBNC cells to sonication was enhanced. These results further confirmed VBNC state was a self-protection mechanism for some bacteria, which minimized cellular energetic requirements and increased the cell resistance. When incubated in tryptic soy broth at 37°C, the VBNC cells induced by HPCD treatment at 25°C, 31°C and 34°C achieved resuscitation, but their resuscitation capabilities decreased with increasing the treatment temperature. Furthermore, electron microscopy revealed changes in the morphology and interior structure of the VBNC cells and the resuscitated cells. These results demonstrated that HPCD could induce E. coli O157:H7 into the VBNC state. Therefore, it is necessary to detect if there exist VBNC microorganisms in HPCD-treated products by molecular-based methods for food safety.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.