ArticlePloS one2013
Reducing AsA leads to leaf lesion and defence response in knock-down of the AsA biosynthetic enzyme GDP-D-mannose pyrophosphorylase gene in tomato plant.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Genome-wide association study reveals the genetic basis of vitamin C content in rapeseed (Frontiers in plant science · 2025Article
- L-Ascorbic acid metabolism and regulation in fruit crops.Plant physiology · 2023Article
- Glucomannan inGenes · 2022Review
- Metabolism and Regulation of Ascorbic Acid in Fruits.Plants (Basel, Switzerland) · 2022Review
- Gene expression profiling inFrontiers in plant science · 2022Article
- Genome-wide identification of GMP genes in Rosaceae and functional characterization of FaGMP4 in strawberry (Fragaria × ananassa).Genes & genomics · 2021Article
- Regulation of Vitamin C Accumulation for Improved Tomato Fruit Quality and Alleviation of Abiotic Stress.Genes · 2021Review
- Sequential Antioxidants Foliar Application Can Alleviate Negative Consequences of Salinity Stress inPlants (Basel, Switzerland) · 2021Article
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- Biosynthetic Gene Pyramiding Leads to Ascorbate Accumulation with Enhanced Oxidative Stress Tolerance in Tomato.International journal of molecular sciences · 2019Article
- Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato.PloS one · 2019Article
- iTRAQ-based quantitative proteomic analysis reveals dynamic changes during daylily flower senescence.Planta · 2018Article
- Molecular Evolution of GDP-D-Mannose Epimerase (Frontiers in plant science · 2018Article
- GDP-D-mannose epimerase regulates male gametophyte development, plant growth and leaf senescence in Arabidopsis.Scientific reports · 2017Article
- Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato.Plant molecular biology · 2016Article
- Rice GDP-mannose pyrophosphorylase OsVTC1-1 and OsVTC1-3 play different roles in ascorbic acid synthesis.Plant molecular biology · 2016Article
- Knocking Down the Expression of GMPase Gene OsVTC1-1 Decreases Salt Tolerance of Rice at Seedling and Reproductive Stages.PloS one · 2016Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a vital antioxidant, L-ascorbic acid (AsA) affects diverse biological processes in higher plants. Lack of AsA in cell impairs plant development. In the present study, we manipulated a gene of GDP-mannose pyrophosphorylase which catalyzes the conversion of D-mannose-1-P to GDP-D-mannose in AsA biosynthetic pathway and found out the phenotype alteration of tomato. In the tomato genome, there are four members of GMP gene family and they constitutively expressed in various tissues in distinct expression patterns. As expected, over-expression of SlGMP3 increased total AsA contents and enhanced the tolerance to oxidative stress in tomato. On the contrary, knock-down of SlGMP3 significantly decreased AsA contents below the threshold level and altered the phenotype of tomato plants with lesions and further senescence. Further analysis indicated the causes for this symptom could result from failing to instantly deplete the reactive oxygen species (ROS) as decline of free radical scavenging activity. More ROS accumulated in the leaves and then triggered expressions of defence-related genes and mimic symptom occurred on the leaves similar to hypersensitive responses against pathogens. Consequently, the photosynthesis of leaves was dramatically fallen. These results suggested the vital roles of AsA as an antioxidant in leaf function and defence response of tomato.
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