ArticleNucleic acids research2013
Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Deep mutational scanning identifies Cas1 and Cas2 variants that enhance type II-A CRISPR-Cas spacer acquisition.Nature communications · 2025Article
- CRISPR-Cas Systems: A Functional Perspective and Innovations.International journal of molecular sciences · 2025Review
- Molecular Details of DNA Integration by CRISPR-Associated Proteins During Adaptation in Bacteria and Archaea.Advances in experimental medicine and biology · 2023Review
- Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair.Frontiers in microbiology · 2022Article
- Digging into the lesser-known aspects of CRISPR biology.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- A CRISPR-Cas9-integrase complex generates precise DNA fragments for genome integration.Nucleic acids research · 2021Article
- Comprehensive classification of ABC ATPases and their functional radiation in nucleoprotein dynamics and biological conflict systems.Nucleic acids research · 2020Review
- Complete Genome Sequence and Carbohydrates-Active EnZymes (CAZymes) Analysis of Lactobacillus paracasei DTA72, a Potential Probiotic Strain with Strong Capability to Use Inulin.Current microbiology · 2020Article
- CRISPRcasIdentifier: Machine learning for accurate identification and classification of CRISPR-Cas systems.GigaScience · 2020Article
- CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR-Cas system.Nucleic acids research · 2019Article
- Quantifying length-dependent DNA end-binding by nucleoproteins.Biophysical chemistry · 2019Article
- Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.Molecular cell · 2019Article
- Molecular organization of the type II-A CRISPR adaptation module and its interaction with Cas9 via Csn2.Nucleic acids research · 2018Article
- Applications of CRISPR/Cas System to Bacterial Metabolic Engineering.International journal of molecular sciences · 2018Review
- Generation of Bacteriophage-Insensitive Mutants of Streptococcus thermophilus via an Antisense RNA CRISPR-Cas Silencing Approach.Applied and environmental microbiology · 2018Article
- Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria.Nature communications · 2017Article
- Protecting genome integrity during CRISPR immune adaptation.Nature structural & molecular biology · 2016Article
- An updated evolutionary classification of CRISPR-Cas systems.Nature reviews. Microbiology · 2015Review
- Global transcription of CRISPR loci in the human oral cavity.BMC genomics · 2015Article
- Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adaptive immunity of bacteria against foreign nucleic acids, mediated by CRISPR (clustered regularly interspaced short palindromic repeats), relies on the specific incorporation of short pieces of the invading foreign DNA into a special genomic locus, termed CRISPR array. The stored sequences (spacers) are subsequently used in the form of small RNAs (crRNAs) to interfere with the target nucleic acid. We explored the DNA-binding mechanism of the immunization protein Csn2 from the human pathogen Streptococcus agalactiae using different biochemical techniques, atomic force microscopic imaging and molecular dynamics simulations. The results demonstrate that the ring-shaped Csn2 tetramer binds DNA ends through its central hole and slides inward, likely by a screw motion along the helical path of the enclosed DNA. The presented data indicate an accessory function of Csn2 during integration of exogenous DNA by end-joining.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.