ArticleJournal of bacteriology2013
Investigating the functional hierarchy of Bacillus megaterium PV361 spore germinant receptors.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Resistance and germination of spores ofJournal of bacteriology · 2025Article
- Revisiting bacterial spore germination in the presence of peptidoglycan fragments.Journal of bacteriology · 2025Article
- Germination ofJournal of bacteriology · 2025Article
- Characterization of germinant receptors ofCurrent research in microbial sciences · 2025Article
- Genomic identification of germinant receptors and phenotypic response to germinants inCurrent research in microbial sciences · 2025Article
- HumanCurrent research in food science · 2024Article
- Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.Journal of bacteriology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Orthologues of Bacillus subtilis Spore Crust Proteins Have a Structural Role in the Bacillus megaterium QM B1551 Spore Exosporium.Applied and environmental microbiology · 2018Article
- Analysis of killing of growing cells and dormant and germinated spores of Bacillus species by black silicon nanopillars.Scientific reports · 2017Article
- Changes in Bacillus Spore Small Molecules, rRNA, Germination, and Outgrowth after Extended Sublethal Exposure to Various Temperatures: Evidence that Protein Synthesis Is Not Essential for Spore Germination.Journal of bacteriology · 2016Article
- The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores.Applied and environmental microbiology · 2016Article
- Structure-function analysis of the Bacillus megaterium GerUD spore germinant receptor protein.FEMS microbiology letters · 2015Article
- Plasmid-encoded genes influence exosporium assembly and morphology in Bacillus megaterium QM B1551 spores.FEMS microbiology letters · 2015Article
- Analysis of metabolism in dormant spores of Bacillus species by 31P nuclear magnetic resonance analysis of low-molecular-weight compounds.Journal of bacteriology · 2015Article
- Article
- Germination of spores of Bacillus species: what we know and do not know.Journal of bacteriology · 2014Review
- Identification of new proteins that modulate the germination of spores of bacillus species.Journal of bacteriology · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Spores of Bacillus megaterium QM B1551 germinate rapidly when exposed to a number of single-trigger germinant compounds, including glucose, proline, leucine, and certain inorganic salts. However, spores of strain PV361, a plasmidless QM B1551 derivative that lacks the GerU germinant receptor (GR) responsible for mediating germination in response to single-trigger compounds, can germinate efficiently when incubated in nutritionally rich media, presumably via activation of additional germinant receptors. In this work, we have identified five chromosomally encoded GRs and attempted to characterize, by mutational analysis, germinant recognition profiles associated with the respective receptors in strain PV361. Of strains engineered with single GR insertion-deletions, only GerK-null spores displayed significant defective germination phenotypes when incubated in 5% (wt/vol) beef extract or plated on rich solid medium. Cumulative decreases in viability were observed in GerK-null spores that also lacked GerA or GerA2, indicating that these GRs, which exerted little effect on spore germination when disrupted individually, have a degree of functionality. Unexpectedly, an efficient germination response to combinations of germinants was restored in GerA(+) spores, which lack all other functional GRs, providing evidence for negative cooperativity between some GRs within the spore. Tetrazolium-based germinative assays conducted with purified spores indicated that these newly characterized B. megaterium GRs are cognate for a wide and chemically diverse range of germinant molecules, but unlike GerU, can only be induced to trigger germination when stimulated by at least two different germinants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.