ArticleChembiochem : a European journal of chemical biology2013
Multiple site-selective insertions of noncanonical amino acids into sequence-repetitive polypeptides.
Article in Chembiochem : a European journal of chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins.Molecules (Basel, Switzerland) · 2023Review
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Minimizing the Anticodon-Recognized Loop ofBiomolecules · 2023Article
- Application of Thermoresponsive Intrinsically Disordered Protein Polymers in Nanostructured and Microstructured Materials.Macromolecular bioscience · 2021Review
- Reprogramming the genetic code.Nature reviews. Genetics · 2021Review
- Engineering the Architecture of Elastin-Like Polypeptides: From Unimers to Hierarchical Self-Assembly.Advanced therapeutics · 2020Article
- Protein-Engineered Functional Materials.Advanced healthcare materials · 2019Review
- Translational Control using an Expanded Genetic Code.International journal of molecular sciences · 2019Review
- Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.Nature communications · 2018Article
- Expanding and reprogramming the genetic code.Nature · 2017Review
- Two-Tier Screening Platform for Directed Evolution of Aminoacyl-tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency.Chembiochem : a European journal of chemical biology · 2017Article
- Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids.Nature biotechnology · 2015Article
- Opportunities for multicomponent hybrid hydrogels in biomedical applications.Biomacromolecules · 2015Review
- Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1.Journal of the American Chemical Society · 2014Article
- Genetic code expansion as a tool to study regulatory processes of transcription.Frontiers in chemistry · 2014Review
- Production of protein-based polymers in Pichia pastoris.Biotechnology advancesReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
A simple and efficient method is described for the introduction of noncanonical amino acids at multiple, defined sites within recombinant polypeptide sequences. Escherichia coli MRA30, a bacterial host strain with attenuated activity of release factor 1 (RF1), was assessed for its ability to support incorporation of a diverse range of noncanonical amino acids in response to multiple encoded amber (TAG) codons within genes derived from superfolder GFP and an elastin-mimetic protein polymer. Suppression efficiency and protein yield depended on the identity of the orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair and the noncanonical amino acid. Elastin-mimetic protein polymers were prepared in which noncanonical amino acid derivatives were incorporated at up to 22 specific sites within the polypeptide sequence with high substitution efficiency. The identities and positions of the variant residues were confirmed by mass spectrometric analysis of the full-length polypeptides and proteolytic cleavage fragments from thermolysin digestion. The data suggest that this multisite suppression approach permits the preparation of protein-based materials in which novel chemical functionalities can be introduced at precisely defined positions within the polypeptide sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.