ArticleBioEssays : news and reviews in molecular, cellular and developmental biology2013
Studying protein-reconstituted proteoliposome fusion with content indicators in vitro.
Article in BioEssays : news and reviews in molecular, cellular and developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Highly stable planar asymmetric suspended membranes for investigating protein dynamics and membrane fusion.Nature protocols · 2025Review
- A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.Current research in physiology · 2024Article
- Neutral lysophosphatidylcholine mediates α-synuclein-induced synaptic vesicle clustering.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Single-vesicle measurement of protein-induced membrane tethering.Colloids and surfaces. B, Biointerfaces · 2019Article
- Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion.Frontiers in molecular neuroscience · 2017Review
- Membrane Fusion Involved in Neurotransmission: Glimpse from Electron Microscope and Molecular Simulation.Frontiers in molecular neuroscience · 2017Review
- Neurodegenerative Disease Related Proteins Have Negative Effects on SNARE-Mediated Membrane Fusion in Pathological Confirmation.Frontiers in molecular neuroscience · 2017Article
- SNARE-mediated membrane fusion in autophagy.Seminars in cell & developmental biology · 2016Review
- C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- N-terminal domain of complexin independently activates calcium-triggered fusion.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.Physical biology · 2015Article
- Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin.The Journal of biological chemistry · 2015Article
- Molecular origins of synaptotagmin 1 activities on vesicle docking and fusion pore opening.Scientific reports · 2015Article
- Reconstituting SNARE-mediated membrane fusion at the single liposome level.Methods in cell biology · 2015Article
- Article
- Nonaggregated α-synuclein influences SNARE-dependent vesicle docking via membrane binding.Biochemistry · 2014Article
- Complexin-1 enhances the on-rate of vesicle docking via simultaneous SNARE and membrane interactions.Journal of the American Chemical Society · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
In vitro reconstitution assays are commonly used to study biological membrane fusion. However, to date, most ensemble and single-vesicle experiments involving SNARE proteins have been performed only with lipid-mixing, but not content-mixing indicators. Through simultaneous detection of lipid and small content-mixing indicators, we found that lipid mixing often occurs seconds prior to content mixing, or without any content mixing at all, during a 50-seconds observation period, for Ca(2+) -triggered fusion with SNAREs, full-length synaptotagmin-1, and complexin. Our results illustrate the caveats of commonly used bulk lipid-mixing fusion experiments. We recommend that proteoliposome fusion experiments should always employ content-mixing indicators in addition to, or in place of, lipid-mixing indicators.
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Registered trials
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