ArticleMethods in molecular biology (Clifton, N.J.)2013
A novel approach to quantify G-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Characterizing ghrelin's role in dysregulating GLP-1-mediated function and promoting the development of alcohol-associated liver disease.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2026Article
- Size Exclusion Chromatography with Multi-Angle Light Scattering for Detergent-Solubilized Integral Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Computationally designed GPCR quaternary structures bias signaling pathway activation.Nature communications · 2022Article
- Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies.Scientific reports · 2022Article
- Functional anatomy of the full-length CXCR4-CXCL12 complex systematically dissected by quantitative model-guided mutagenesis.Science signaling · 2020Article
- Common activation mechanism of class A GPCRs.eLife · 2019Article
- Using the novel HiBiT tag to label cell surface relaxin receptors for BRET proximity analysis.Pharmacology research & perspectives · 2019Article
- Protease-activated receptor-4 and purinergic receptor P2Y12 dimerize, co-internalize, and activate Akt signaling via endosomal recruitment of β-arrestin.The Journal of biological chemistry · 2017Article
- BRET evidence that β2 adrenergic receptors do not oligomerize in cells.Scientific reports · 2015Article
- Stoichiometry and geometry of the CXC chemokine receptor 4 complex with CXC ligand 12: molecular modeling and experimental validation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Improved methodical approach for quantitative BRET analysis of G Protein Coupled Receptor dimerization.PloS one · 2014Article
- Chemokine receptor oligomerization and allostery.Progress in molecular biology and translational science · 2013Review
- Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Along with other resonance energy transfer techniques, bioluminescence resonance energy transfer (BRET) has emerged as an important method for demonstrating protein-protein interactions in cells. In the field of G-protein-coupled receptors, including chemokine receptors, BRET has been widely used to investigate homo- and heterodimerization, a feature of their interactions that is emerging as integral to function and regulation. While demonstrating the existence of dimers for a given receptor proved to be fairly straightforward, quantitative comparisons of different receptors or mutants are nontrivial because of inevitable variations in the expression of receptor constructs. The uncontrollable parameters of the cellular expression machinery make amounts of transfected DNA extremely poor predictors for the expression levels of BRET donor and acceptor receptor constructs, even in relative terms. In this chapter, we show that properly accounting for receptor expression levels is critical for quantitative interpretation of BRET data. We also provide a comprehensive account of expected responses in all types of BRET experiments and propose a framework for uniform and accurate quantitative treatment of these responses. The framework allows analysis of both homodimer and heterodimer BRET data. The important caveats and obstacles for quantitative treatment are outlined, and the utility of the approach is illustrated by its application to the homodimerization of wild-type (WT) and mutant forms of the chemokine receptor CXCR4.
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