Evidence map›Paper›PMID 23625462›Full record

ArticleJournal of molecular medicine (Berlin, Germany)2013

MicroRNA-1 enhances the angiogenic differentiation of human cardiomyocyte progenitor cells.

Alain van Mil, Krijn R Vrijsen, Marie-José Goumans, Corina H Metz, Pieter A Doevendans, Joost P Sluijter

Abstract read
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In one paragraph

Article in Journal of molecular medicine (Berlin, Germany), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
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  4. Review
  5. Article
  6. Review
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  8. Article
  9. Article
  10. Article
  11. Regulatory non-coding RNAs in acute myocardial infarction.Journal of cellular and molecular medicine · 2017
    Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. The Role of MicroRNAs in Cardiac Stem Cells.Stem cells international · 2015
    Review
  18. Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alain van MilDepartment of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht, Heidelberglaan 100, room G02.523, 3584 CX, Utrecht, The Netherlands.
Krijn R Vrijsen
Marie-José Goumans
Corina H Metz
Pieter A Doevendans
Joost P Sluijter

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Instigated by the discovery of adult cardiac progenitor cells, cell replacement therapy has become a promising option for myocardial repair in the past decade. We have previously shown that human-derived cardiomyocyte progenitor cells (hCMPCs) can differentiate into cardiomyocyte-, endothelial-, and smooth muscle-like cells in vitro, and in vivo after transplantation in a mouse model of myocardial infarction, resulting in preservation of cardiac function. However, to allow successful repopulation of the injured myocardium, it is of key importance to restore myocardial perfusion by the formation of new vasculature. Several studies have shown that microRNAs regulate vascular differentiation of different stem/progenitor cells. Here, we show that miR-1 is upregulated in hCMPCs during angiogenic differentiation. Upregulation of miR-1 enhanced the formation of vascular tubes on Matrigel and within a collagen matrix, and also increased hCMPC motility, as shown by planar and transwell migration assays. By western blot, qRT-PCR and luciferase reporter assays, miR-1 was found to directly target and inhibit the expression of sprouty-related EVH1 domain-containing protein 1 (Spred1). Knocking down Spred1 phenocopies the functional effect seen for miR-1 upregulation. Using a systems biology approach, we found that in hCMPCs, miR-1 is proposed to control a network of genes predominantly involved in angiogenesis-related processes, including the Spred1 pathway. Our data shows that by upregulation of miR-1, the angiogenic differentiation of hCMPCs can be enhanced, which may be used as a new therapeutic approach to improve the efficiency of cell-based therapy for cardiac regeneration by enhancing the formation of new vasculature.

Indexed as

Adaptor Proteins, Signal TransducingCell DifferentiationCell MovementCells, CulturedHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMicroRNAsMyocytes, CardiacNeovascularization, PhysiologicStem CellsAdaptor Proteins, Signal TransducingIntracellular Signaling Peptides and ProteinsMembrane ProteinsMicroRNAsMIRN1 microRNA, humanSPRED1 protein, human

Identifiers

PMID23625462

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.