ArticleScientific reports2013
Quantitative transcriptomics using designed primer-based amplification.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- The recent advancements of single-cell RNA sequencing in pre-eclampsia.Frontiers in medicine · 2026Review
- Review
- Non-coding RNAs in human health and disease: potential function as biomarkers and therapeutic targets.Functional & integrative genomics · 2023Review
- The impact of single-cell genomics on the field of mycobacterial infection.Frontiers in microbiology · 2022Review
- Toward uncharted territory of cellular heterogeneity: advances and applications of single-cell RNA-seq.Journal of translational genetics and genomics · 2021Article
- Principles and innovative technologies for decrypting noncoding RNAs: from discovery and functional prediction to clinical application.Journal of hematology & oncology · 2020Review
- Non-coding RNAs in cancer: platforms and strategies for investigating the genomic "dark matter".Journal of experimental & clinical cancer research : CR · 2020Review
- Tumour heterogeneity and metastasis at single-cell resolution.Nature cell biology · 2018Review
- Global and targeted approaches to single-cell transcriptome characterization.Briefings in functional genomics · 2018Article
- Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing.Clinical chemistry · 2018Article
- Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.Nucleic acids research · 2018Review
- Single-cell RNA sequencing to explore immune cell heterogeneity.Nature reviews. Immunology · 2018Review
- Robust transcriptional signatures for low-input RNA samples based on relative expression orderings.BMC genomics · 2017Article
- RNA-Seq methods for transcriptome analysis.Wiley interdisciplinary reviews. RNA · 2017Review
- Accurate Profiling of Gene Expression and Alternative Polyadenylation with Whole Transcriptome Termini Site Sequencing (WTTS-Seq).Genetics · 2016Article
- Nuclear RNA-seq of single neurons reveals molecular signatures of activation.Nature communications · 2016Article
- Article
- Single-cell transcriptome sequencing: recent advances and remaining challenges.F1000Research · 2016Review
- Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- A comparison of sperm RNA-seq methods.Systems biology in reproductive medicine · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We developed a novel Designed Primer-based RNA-sequencing strategy (DP-seq) that uses a defined set of heptamer primers to amplify the majority of expressed transcripts from limiting amounts of mRNA, while preserving their relative abundance. Our strategy reproducibly yielded high levels of amplification from as low as 50 picograms of mRNA while offering a dynamic range of over five orders of magnitude in RNA concentrations. We also demonstrated the potential of DP-seq to selectively suppress the amplification of the highly expressing ribosomal transcripts by more than 70% in our sequencing library. Using lineage segregation in embryonic stem cell cultures as a model of early mammalian embryogenesis, DP-seq revealed novel sets of low abundant transcripts, some corresponding to the identity of cellular progeny before they arise, reflecting the specification of cell fate prior to actual germ layer segregation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.