ArticleCell cycle (Georgetown, Tex.)2013
A non-canonical mode of microtubule organization operates throughout pre-implantation development in mouse.
Article in Cell cycle (Georgetown, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Skeletal muscle dedifferentiation requires centrosomes.NPJ Regenerative medicine · 2026Article
- Developmental and tissue-specific roles of mammalian centrosomes.The FEBS journal · 2025Review
- The evolution of centriole degradation in mouse sperm.Nature communications · 2024Article
- The centrosome - diverse functions in fertilization and development across species.Journal of cell science · 2023Review
- Omics Views of Mechanisms for Cell Fate Determination in Early Mammalian Development.Genomics, proteomics & bioinformatics · 2023Review
- Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.The EMBO journal · 2023Article
- A monoastral mitotic spindle determines lineage fate and position in the mouse embryo.Nature cell biology · 2022Article
- The human sperm basal body is a complex centrosome important for embryo preimplantation development.Molecular human reproduction · 2021Article
- Cytoskeletal control of early mammalian development.Nature reviews. Molecular cell biology · 2021Review
- Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.EMBO reports · 2021Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- Tetraploidy causes chromosomal instability in acentriolar mouse embryos.Nature communications · 2019Article
- HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes.Cell division · 2019Article
- Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse.Scientific reports · 2018Article
- Once and only once: mechanisms of centriole duplication and their deregulation in disease.Nature reviews. Molecular cell biology · 2018Review
- Tetraploidy-associated centrosome overduplication in mouse early embryos.Communicative & integrative biology · 2018Article
- Tri-directional anaphases as a novel chromosome segregation defect in human oocytes.Human reproduction (Oxford, England) · 2017Article
- Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation.Scientific reports · 2016Article
- On becoming accepting of the imperfectionsin mammalian embryogenesis.Journal of assisted reproduction and genetics · 2016Article
- Nucleus downscaling in mouse embryos is regulated by cooperative developmental and geometric programs.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In dividing animal cells, the centrosome, comprising centrioles and surrounding pericentriolar-material (PCM), is the major interphase microtubule-organizing center (MTOC), arranging a polarized array of microtubules (MTs) that controls cellular architecture. The mouse embryo is a unique setting for investigating the role of centrosomes in MT organization, since the early embryo is acentrosomal, and centrosomes emerge de novo during early cleavages. Here we use embryos from a GFP::CETN2 transgenic mouse to observe the emergence of centrosomes and centrioles in embryos, and show that unfocused acentriolar centrosomes first form in morulae (~16-32-cell stage) and become focused at the blastocyst stage (~64-128 cells) concomitant with the emergence of centrioles. We then used high-resolution microscopy and dynamic tracking of MT growth events in live embryos to examine the impact of centrosome emergence upon interphase MT dynamics. We report that pre-implantation mouse embryos of all stages employ a non-canonical mode of MT organization that generates a complex array of randomly oriented MTs that are preferentially nucleated adjacent to nuclear and plasmalemmal membranes and cell-cell interfaces. Surprisingly, however, cells of the early embryo continue to employ this mode of interphase MT organization even after the emergence of centrosomes. Centrosomes are found at MT-sparse sites and have no detectable impact upon interphase MT dynamics. To our knowledge, the early embryo is unique among proliferating cells in adopting an acentrosomal mode of MT organization despite the presence of centrosomes, revealing that the transition to a canonical mode of interphase MT organization remains incomplete prior to implantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.