ArticleApplied and environmental microbiology2013
Inactivation of Ca(2+)/H(+) exchanger in Synechocystis sp. strain PCC 6803 promotes cyanobacterial calcification by upregulating CO(2)-concentrating mechanisms.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Operando Microscopy of Photosynthetic Microbial Biohybrids Using Fluorescent Chemical Probes.ACS electrochemistry · 2026Article
- Microbial Carbonate Mineralization: A Comprehensive Review of Mechanisms, Applications, and Recent Advancements.Molecular biotechnology · 2026Review
- Ecological, Functional, and Phylogenetic Determinants of Cyanobacterial Biomineralisation.Environmental microbiology reports · 2026Review
- Applications of bio-enhanced cementitious composites for sustainable building materials: emission-reduction and carbon-sequestration pathways.Systems microbiology and biomanufacturing · 2026Review
- Calcite precipitation by the nitrogen-fixing cyanobacterium Anabaena sp. ATCC 33047.Communications biology · 2025Article
- Photobiocatalytic Oxyfunctionalization with High Reaction Rate using a Baeyer-Villiger Monooxygenase fromACS catalysis · 2022Article
- CyanobacteriumBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- Sycrp2 Is Essential for Twitching Motility in the Cyanobacterium Synechocystis sp. Strain PCC 6803.Journal of bacteriology · 2018Article
- Outer Membrane Iron Uptake Pathways in the Model Cyanobacterium Synechocystis sp. Strain PCC 6803.Applied and environmental microbiology · 2018Article
- Amorphous Calcium Carbonate Granules Form Within an Intracellular Compartment in Calcifying Cyanobacteria.Frontiers in microbiology · 2018Article
- Co-ordination of NDH and Cup proteins in CO2 uptake in cyanobacterium Synechocystis sp. PCC 6803.Journal of experimental botany · 2017Article
- Environmental pH and the Requirement for the Extrinsic Proteins of Photosystem II in the Function of Cyanobacterial Photosynthesis.Frontiers in plant science · 2016Article
- Carbonate Precipitation through Microbial Activities in Natural Environment, and Their Potential in Biotechnology: A Review.Frontiers in bioengineering and biotechnology · 2016Review
- Article
- Intracellular Ca-carbonate biomineralization is widespread in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Acclimation of Synechocystis sp. PCC 6803 to Alkaline pH Under Ambient Air.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are important players in the global carbon cycle, accounting for approximately 25% of global CO2 fixation. Their CO2-concentrating mechanisms (CCMs) are thought to play a key role in cyanobacterial calcification, but the mechanisms are not completely understood. In Synechocystis sp. strain PCC 6803, a single Ca(2+)/H(+) exchanger (Slr1336) controls the Ca(2+)/H(+) exchange reaction. We knocked out the exchanger and investigated the effects on cyanobacterial calcification and CCMs. Inactivation of slr1336 significantly increased the calcification rate and decreased the zeta potential, indicating a relatively stronger Ca(2+)-binding ability. Some genes encoding CCM-related components showed increased expression levels, including the cmpA gene, which encodes the Ca(2+)-dependent HCO3(-) transporter BCT1. The transcript level of cmpA in the mutant was 30 times that in wild type. A Western blot analysis further confirmed that protein levels of CmpA were higher in the mutant than the wild type. Measurements of inorganic carbon fluxes and O2 evolution proved that both the net HCO3(-) uptake rate and the BCT1 transporter supported photosynthetic rate in the slr1336 mutant were significantly higher than in the wild type. This would cause the mutant cells to liberate more OH(-) ions out of the cell and stimulate CaCO3 precipitation in the microenvironment. We conclude that the mutation of the Ca(2+)/H(+) exchanger in Synechocystis promoted the cyanobacterial calcification process by upregulating CCMs, especially the BCT1 HCO3(-) transporter. These results shed new light on the mechanism by which CCM-facilitated photosynthesis promotes cyanobacterial calcification.
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