ArticleNature chemical biology2013
Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis.
Article in Nature chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 1 citations in OpenAlex.
- Identification of the D-glucuronyl C5-epimerase that introduces iduronic acid into N-linked glycans decorating archaeal glycoproteins.Communications biology · 2025Article
- Article
- N-glycosylation in Archaea - Expanding the process, components and roles of a universal post-translational modification.BBA advances · 2024Article
- Agl24 is an ancient archaeal homolog of the eukaryotic N-glycan chitobiose synthesis enzymes.eLife · 2022Article
- The structure of an archaeal oligosaccharyltransferase provides insight into the strict exclusion of proline from the N-glycosylation sequon.Communications biology · 2021Article
- Identifying Components of aFrontiers in microbiology · 2021Article
- Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces.Current opinion in structural biology · 2019Review
- A dual-chain assembly pathway generates the high structural diversity of cell-wall polysaccharides inThe Journal of biological chemistry · 2019Article
- In Search for the Membrane Regulators of Archaea.International journal of molecular sciences · 2019Review
- Disrupted Synthesis of a Di-Journal of bacteriology · 2019Article
- Biogenesis of Asparagine-Linked Glycoproteins Across Domains of Life-Similarities and Differences.ACS chemical biology · 2018Article
- Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases.Trends in biochemical sciences · 2018Review
- Bacterial phosphoglycosyl transferases: initiators of glycan biosynthesis at the membrane interface.Glycobiology · 2017Review
- Transmembrane motions of PglB induced by LLO are coupled with EL5 loop conformational changes necessary for OST activity.Glycobiology · 2017Article
- Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.Methods in enzymology · 2017Article
- AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7.Extremophiles : life under extreme conditions · 2017Article
- Epigenetic regulation of glycosylation and the impact on chemo-resistance in breast and ovarian cancer.Epigenetics · 2016Review
- N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.FEBS letters · 2016Article
- The multiple evolutionary origins of the eukaryotic N-glycosylation pathway.Biology direct · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Asparagine-linked glycosylation is a complex protein modification conserved among all three domains of life. Herein we report the in vitro analysis of N-linked glycosylation from the methanogenic archaeon Methanococcus voltae. Using a suite of synthetic and semisynthetic substrates, we show that AglK initiates N-linked glycosylation in M. voltae through the formation of α-linked dolichyl monophosphate N-acetylglucosamine, which contrasts with the polyprenyl diphosphate intermediates that feature in both eukaryotes and bacteria. Notably, AglK has high sequence homology to dolichyl phosphate β-glucosyltransferases, including Alg5 in eukaryotes, suggesting a common evolutionary origin. The combined action of the first two enzymes, AglK and AglC, afforded an α-linked dolichyl monophosphate glycan that serves as a competent substrate for the archaeal oligosaccharyl transferase AglB. These studies provide what is to our knowledge the first biochemical evidence revealing that, despite the apparent similarity of the overall pathways, there are actually two general strategies to achieve N-linked glycoproteins across the domains of life.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.