ArticleMethods (San Diego, Calif.)2013
Multi-allelic phenotyping--a systematic approach for the simultaneous analysis of multiple induced mutations.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Disruption ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Pain management in zebrafish : Report from a FELASA Working Group.Laboratory animals · 2024Review
- Digenic inheritance involving a muscle-specific protein kinase and the giant titin protein causes a skeletal muscle myopathy.Nature genetics · 2024Article
- The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo.Development (Cambridge, England) · 2022Article
- Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?Cryobiology · 2022Article
- Evolution of the potassium channel gene Kcnj13 underlies colour pattern diversification in Danio fish.Nature communications · 2020Article
- Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish.Nature communications · 2019Article
- The gene regulatory basis of genetic compensation during neural crest induction.PLoS genetics · 2019Article
- Zebrafish Models of Neurodevelopmental Disorders: Limitations and Benefits of Current Tools and Techniques.International journal of molecular sciences · 2019Review
- Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.PLoS genetics · 2018Article
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- Loss of the chromatin modifier Kdm2aa causes BrafV600E-independent spontaneous melanoma in zebrafish.PLoS genetics · 2017Article
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- The age of heterozygousWellcome open research · 2017Article
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- Article
- Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.BMC genomics · 2016Article
- The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish.PLoS genetics · 2015Article
- High-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenotyping.BMC genomics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The zebrafish mutation project (ZMP) aims to generate a loss of function allele for every protein-coding gene, but importantly to also characterise the phenotypes of these alleles during the first five days of development. Such a large-scale screen requires a systematic approach both to identifying phenotypes, and also to linking those phenotypes to specific mutations. This phenotyping pipeline simultaneously assesses the consequences of multiple alleles in a two-step process. First, mutations that do not produce a visible phenotype during the first five days of development are identified, while a second round of phenotyping focuses on detailed analysis of those alleles that are suspected to cause a phenotype. Allele-specific PCR single nucleotide polymorphism (SNP) assays are used to genotype F2 parents and individual F3 fry for mutations known to be present in the F1 founder. With this method specific phenotypes can be linked to induced mutations. In addition a method is described for cryopreserving sperm samples of mutagenised males and their subsequent use for in vitro fertilisation to generate F2 families for phenotyping. Ultimately this approach will lead to the functional annotation of the zebrafish genome, which will deepen our understanding of gene function in development and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.