ArticleStructure (London, England : 1993)2013
Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli.
Article in Structure (London, England : 1993), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 82 citations in OpenAlex.
- Wzi modulates immune evasion of hypervirulentVirulence · 2026Article
- Capsular Polysaccharide Safeguards a Prophage-Bacterium Symbiosis by Preventing Collateral Attack and Promoting Viral Transmission.bioRxiv : the preprint server for biology · 2026Article
- Exploring glycocalyx components powering the contact between Entamoeba histolytica and bacteria during non-opsonic phagocytosis.PLoS pathogens · 2026Review
- GMMA as delivery system for Klebsiella pneumoniae O-antigens and K-antigens.Scientific reports · 2026Article
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026Review
- Overcoming phagocytosis resistance of hypervirulentMicrobial cell (Graz, Austria) · 2026Article
- O-antigen polysaccharides inMicrobiology and molecular biology reviews : MMBR · 2025Review
- MDTAP: a tool to analyze permeation events across membrane proteins.Bioinformatics advances · 2025Article
- Insights into the recognition of hypermucoviscousFrontiers in immunology · 2024Article
- Urine-mediated suppression ofmSphere · 2023Article
- The making of hypervirulent Klebsiella pneumoniae.Journal of clinical laboratory analysis · 2022Review
- What Glues the Glue to the Cell Surface?Journal of bacteriology · 2022Article
- HfaE Is a Component of the Holdfast Anchor Complex That Tethers the Holdfast Adhesin to the Cell Envelope.Journal of bacteriology · 2022Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Article
- LPS O Antigen Plays a Key Role in Klebsiella pneumoniae Capsule Retention.Microbiology spectrum · 2022Article
- Weaving of bacterial cellulose by the Bcs secretion systems.FEMS microbiology reviews · 2022Review
- Identification of distinct capsule types associated with Serratia marcescens infection isolates.PLoS pathogens · 2022Article
- Article
- The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface.Scientific reports · 2021Article
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6 authors at 3 institutions in 2 countries.
Funding
Abstract
Many pathogenic bacteria encase themselves in a polysaccharide capsule that provides a barrier to the physical and immunological challenges of the host. The mechanism by which the capsule assembles around the bacterial cell is unknown. Wzi, an integral outer-membrane protein from Escherichia coli, has been implicated in the formation of group 1 capsules. The 2.6 Å resolution structure of Wzi reveals an 18-stranded β-barrel fold with a novel arrangement of long extracellular loops that blocks the extracellular entrance and a helical bundle that plugs the periplasmic end. Mutagenesis shows that specific extracellular loops are required for in vivo capsule assembly. The data show that Wzi binds the K30 carbohydrate polymer and, crucially, that mutants functionally deficient in vivo show no binding to K30 polymer in vitro. We conclude that Wzi is a novel outer-membrane lectin that assists in the formation of the bacterial capsule via direct interaction with capsular polysaccharides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.