ArticleCurrent biology : CB2013
The budding yeast point centromere associates with two Cse4 molecules during mitosis.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 50 citations in OpenAlex.
- Direct observation of coordinated assembly of individual native centromeric nucleosomes.The EMBO journal · 2023Article
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.Science advances · 2023Article
- Direct observation of coordinated assembly of individual native centromeric nucleosomes.bioRxiv : the preprint server for biology · 2023Article
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.Current opinion in cell biology · 2019Review
- Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.SLAS technology · 2019Article
- An asymmetric centromeric nucleosome.eLife · 2018Article
- An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.eLife · 2018Article
- Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.Science (New York, N.Y.) · 2018Article
- Structural plasticity of the living kinetochore.The Journal of cell biology · 2017Article
- How Kinetochore Architecture Shapes the Mechanisms of Its Function.Current biology : CB · 2017Review
- Article
- An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites.eLife · 2017Article
- A Cell Biological Perspective on Past, Present and Future Investigations of the Spindle Assembly Checkpoint.Biology · 2016Review
- Dual mechanisms regulate the recruitment of spindle assembly checkpoint proteins to the budding yeast kinetochore.Molecular biology of the cell · 2016Article
- Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.PLoS genetics · 2016Article
- Fluorescent foci quantitation for high-throughput analysis.Journal of biological methods · 2015Article
- The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.Nature cell biology · 2015Article
- Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies.Journal of molecular biology · 2015Review
- Multisite phosphorylation of the NDC80 complex gradually tunes its microtubule-binding affinity.Molecular biology of the cell · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The centromere is defined by the incorporation of the centromere-specific histone H3 variant centromere protein A (CENP-A). Like histone H3, CENP-A can form CENP-A-H4 heterotetramers in vitro. However, the in vivo conformation of CENP-A chromatin has been proposed by different studies as hemisomes, canonical, or heterotypic nucleosomes. A clear understanding of the in vivo architecture of CENP-A chromatin is important, because it influences the molecular mechanisms of the assembly and maintenance of the centromere and its function in kinetochore nucleation. A key determinant of this architecture is the number of CENP-A molecules bound to the centromere. Accurate measurement of this number can limit possible centromere architectures. The genetically defined point centromere in the budding yeast Saccharomyces cerevisiae provides a unique opportunity to define this number accurately, as this 120-bp-long centromere can at the most form one nucleosome or hemisome. Using novel live-cell fluorescence microscopy assays, we demonstrate that the budding yeast centromere recruits two Cse4 (ScCENP-A) molecules. These molecules are deposited during S phase and they remain stably bound through late anaphase. Our studies suggest that the budding yeast centromere incorporates a Cse4-H4 tetramer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.