ArticleParasites & vectors2013
Characterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
- A multifaceted molecular approach to surveillance of leishmaniasis: Identification of sand fly species, Leishmania parasites, and blood meal sources using high-resolution melting analysis.PLoS neglected tropical diseases · 2025Article
- Genetic diversity and comparative genomics across Leishmania (Viannia) species.Communications biology · 2025Article
- Isolation and characterisation of Leishmania (Leishmania) infantum from cutaneous leishmaniasis patients in northeast Brazil.Memorias do Instituto Oswaldo Cruz · 2024Article
- Immunosuppressants alter the immune response associated with GlucantimeFrontiers in immunology · 2023Article
- Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites.Communications biology · 2022Review
- Transcriptomic Profile of Canine DH82 Macrophages Infected byInternational journal of molecular sciences · 2022Article
- Article
- Workflow ofInternational journal for parasitology. Parasites and wildlife · 2021Article
- Loop-Mediated Isothermal Amplification Allows Rapid, Simple and Accurate Molecular Diagnosis of Human Cutaneous and Visceral Leishmaniasis Caused byMicroorganisms · 2021Article
- Effect of immunosuppressants on the parasite load developed in, and immune response to, visceral leishmaniasis: A comparative study in a mouse model.PLoS neglected tropical diseases · 2021Article
- Role of asymptomatic and symptomatic humans as reservoirs of visceral leishmaniasis in a Mediterranean context.PLoS neglected tropical diseases · 2020Article
- Article
- Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains.Memorias do Instituto Oswaldo Cruz · 2020Article
- Amazonian Phlebovirus (Bunyaviridae) potentiates the infection of Leishmania (Leishmania) amazonensis: Role of the PKR/IFN1/IL-10 axis.PLoS neglected tropical diseases · 2019Article
- Functional genomics in sand fly-derived Leishmania promastigotes.PLoS neglected tropical diseases · 2019Review
- More than just exosomes: distinctJournal of extracellular vesicles · 2019Article
- Genomic Analysis of Colombian Leishmania panamensis strains with different level of virulence.Scientific reports · 2018Article
- Immunology and Genetic ofJournal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2018Review
- Infectivity of Post-Kala-azar Dermal Leishmaniasis Patients to Sand Flies: Revisiting a Proof of Concept in the Context of the Kala-azar Elimination Program in the Indian Subcontinent.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- IFN-γ, IL-2, IP-10, and MIG as Biomarkers of Exposure toFrontiers in cellular and infection microbiology · 2017Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmaniasis is a group of diseases with a variety of clinical manifestations. The form of the disease is highly dependent on the infective Leishmania species and the immunological status of the host. The infectivity of the parasite strain also plays an important role in the progression of the infection. The aim of this work is to understand the influence of the natural infectivity of Leishmania strains in the outcome of visceral leishmaniasis.
methodsIn this study we have characterized four strains of L. infantum in terms of molecular typing, in vitro cultivation and differentiation. Two strains were isolated from HIV+ patients with visceral leishmaniasis (Bibiano and E390M), one strain was isolated from a cutaneous lesion in an immunocompetent patient (HL) and another internal reference strain causative of visceral leishmaniasis (ST) also from an immunocompetent patient was used for comparison. For this objective, we have compared their virulence by in vitro and in vivo infectivity in a murine model of visceral leishmaniasis.
resultsMolecular typing unraveled a new k26 sequence attributed to MON-284 zymodeme and allowed the generation of a molecular signature for the identification of each strain. In vitro cultivation enabled the production of promastigotes with comparable growth curves and metacyclogenesis development. The HL strain was the most infective, showing the highest parasite loads in vitro that were corroborated with the in vivo assays, 6 weeks post-infection in BALB/c mice. The two strains isolated from HIV+ patients, both belonging to two different zymodemes, revealed different kinetics of infection.
conclusionDifferences in in vitro and in vivo infectivity found in the murine model were then attributed to intrinsic characteristics of each strain. This work is supported by other studies that present the parasite's inherent features as factors for the multiplicity of clinical manifestations and severity of leishmaniasis.
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