ArticleBMC genomics2013
Genome-wide promoter methylation profile of human testis and epididymis: identified from cell-free seminal DNA.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- On the Role of Seminal Fluid Protein and Nucleic Acid Content in Paternal Epigenetic Inheritance.International journal of molecular sciences · 2022Review
- A higher preconceptional paternal body mass index influences fertilization rate and preimplantation embryo development.Andrology · 2022Article
- Cell-free DNA discoveries in human reproductive medicine: providing a new tool for biomarker and genetic assays in ART.Journal of assisted reproduction and genetics · 2021Review
- Epigenetics of Male Infertility: The Role of DNA Methylation.Frontiers in cell and developmental biology · 2021Review
- Impact of Preanalytical and Analytical Methods on Cell-Free DNA Diagnostics.Frontiers in cell and developmental biology · 2021Article
- An Epigenome-Wide DNA Methylation Map of Testis in Pigs for Study of Complex Traits.Frontiers in genetics · 2019Article
- Genome-wide DNA Methylation and RNAseq Analyses Identify Aberrant Signalling Pathways in Focal Cortical Dysplasia (FCD) Type II.Scientific reports · 2018Article
- Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia.Oncotarget · 2017Article
- Collapsed methylation quantitative trait loci analysis for low frequency and rare variants.Human molecular genetics · 2016Article
- Epigenetics: A key paradigm in reproductive health.Clinical and experimental reproductive medicine · 2016Review
- Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).Molecular & cellular proteomics : MCP · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA methylation analysis is useful for investigation of male fertility in mammals, whereas the reliance on tissues limits the research on human. We have previously found the presence of high concentration of cell-free seminal DNA (cfsDNA) in human semen. We proposed that some testis and epididymis-specific methylated promoters could be detected in human cfsDNA, and thus hold promise as noninvasive epigenetic biomarkers for male infertility, of which most cases are caused by defects in testicular sperm production or epididymal sperm maturation.
resultsThe ejaculate of successfully vasectomized men does not contain any secretion from testis and epididymis. Here we compared genome-wide promoter methylation profiles in cfsDNA between health donors and post-vasectomy men. Promoters of 367 testis and epididymis-specific hypomethylated genes and 134 hypermethylated genes were identified. Subsequent validation by Methyl-DNA immunoprecipitation and MethyLight analysis confirmed the result of promoter microarray. Gene Ontology analysis revealed many genes involved in male reproduction.
conclusionWe detected the testis and epididymis-specific methylated promoters in human cfsDNA, which may be used for noninvasive epigenetic biomarkers for the study and diagnosis of male infertility.
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