ArticlePloS one2013
Toxic effect of silica nanoparticles on endothelial cells through DNA damage response via Chk1-dependent G2/M checkpoint.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 189 citations in OpenAlex.
- 3D-Printed Composite Scaffolds Containing SiOACS omega · 2026Review
- Nanotoxicity of Porous Silica Nanoparticles: Physicochemical Properties and Mechanistic Cellular Endpoints.Nanomaterials (Basel, Switzerland) · 2025Review
- Two-Step Air/Water Oxidation Process for the Long-Lasting Photoluminescence and Biological Viability (MTT Assay) of Porous Silicon Particles.Journal of xenobiotics · 2025Article
- Benchmark dose determining airborne crystalline silica particles based on A549 lung-cell line survival in an in vitro study.Scientific reports · 2024Article
- The pivotal role of irradiation-induced apoptosis in the pathogenesis and therapy of medulloblastoma.Cancer reports (Hoboken, N.J.) · 2024Review
- State of the Art of Silica Nanoparticles: An Overview on Biodistribution and Preclinical Toxicity Studies.The AAPS journal · 2024Review
- Cellular Alterations Due to Direct and Indirect Interaction of Nanomaterials with Nucleic Acids.International journal of molecular sciences · 2024Review
- A Spike-like Self-Assembly of Polyaspartamide Integrated with Functionalized Nanoparticles.Polymers · 2024Article
- Variation in pentose phosphate pathway-associated metabolism dictates cytotoxicity outcomes determined by tetrazolium reduction assays.Scientific reports · 2023Article
- The toxicity of nanoparticles and their interaction with cells: anNanoscale advances · 2023Review
- Antibacterial and biofilm-inhibitory effects of vancomycin-loaded mesoporous silica nanoparticles on methicillin-resistant staphylococcus aureus and gram-negative bacteria.Archives of microbiology · 2023Article
- Overview of mechanism and consequences of endothelial leakiness caused by metal and polymeric nanoparticles.Beilstein journal of nanotechnology · 2023Review
- Experimental study on novel biodegradable Zn-Fe-Si alloys.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Chelator-Free Copper-64-Incorporated Iron Oxide Nanoparticles for PET/MR Imaging: Improved Radiocopper Stability and Cell Viability.Nanomaterials (Basel, Switzerland) · 2022Article
- CeOToxics · 2022Article
- Identifying the Molecular Mechanisms and Types of Cell Death Induced byInternational journal of molecular sciences · 2022Article
- Organ-on-a-chip technology for nanoparticle research.Nano convergence · 2021Review
- Toxic Impacts of Amorphous Silica Nanoparticles on Liver and Kidney of Male Adult Rats: an In Vivo Study.Biological trace element research · 2021Article
- Are Biogenic and Pyrogenic Mesoporous SiOMolecules (Basel, Switzerland) · 2021Article
- Global Proteomics to Study Silica Nanoparticle-Induced Cytotoxicity and Its Mechanisms in HepG2 Cells.Biomolecules · 2021Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silica nanoparticles have become promising carriers for drug delivery or gene therapy. Endothelial cells could be directly exposed to silica nanoparticles by intravenous administration. However, the underlying toxic effect mechanisms of silica nanoparticles on endothelial cells are still poorly understood. In order to clarify the cytotoxicity of endothelial cells induced by silica nanoparticles and its mechanisms, cellular morphology, cell viability and lactate dehydrogenase (LDH) release were observed in human umbilical vein endothelial cells (HUVECs) as assessing cytotoxicity, resulted in a dose- and time- dependent manner. Silica nanoparticles-induced reactive oxygen species (ROS) generation caused oxidative damage followed by the production of malondialdehyde (MDA) as well as the inhibition of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Both necrosis and apoptosis were increased significantly after 24 h exposure. The mitochondrial membrane potential (MMP) decreased obviously in a dose-dependent manner. The degree of DNA damage including the percentage of tail DNA, tail length and Olive tail moment (OTM) were markedly aggravated. Silica nanoparticles also induced G2/M arrest through the upregulation of Chk1 and the downregulation of Cdc25C, cyclin B1/Cdc2. In summary, our data indicated that the toxic effect mechanisms of silica nanoparticles on endothelial cells was through DNA damage response (DDR) via Chk1-dependent G2/M checkpoint signaling pathway, suggesting that exposure to silica nanoparticles could be a potential hazards for the development of cardiovascular diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.