Trial reportPloS one2013
Reproducibility of non-invasive assessment of skin endothelial function using laser Doppler flowmetry and laser speckle contrast imaging.
Trial report in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01664572 (Methodological Issues of Laser Speckle Contrast Imaging for Studying Microcirculation.), which is not on this map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Methodological Issues of Laser Speckle Contrast Imaging for Studying Microcirculation.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Vascular effects of biologic and targeted synthetic antirheumatic drugs approved for rheumatoid arthritis: a systematic review.Clinical rheumatology · 2023Pooled it
- Successful improved peripheral tissue perfusion was seen in patients with atherosclerosis after 12 months of treatment with aged garlic extract.International wound journal · 2021Trial
- Neurovascular microcirculatory vasodilation mediated by C-fibers and Transient receptor potential vanilloid-type-1 channels (TRPV 1) is impaired in type 1 diabetes.Scientific reports · 2017Trial
- Evidence for a vasomotor cyclo-oxygenase dependent mechanism of sensitization at the cutaneous level.British journal of clinical pharmacology · 2015Trial
- Non-Invasive Measurement of Skin Microvascular Response during Pharmacological and Physiological Provocations.PloS one · 2015Trial
- The relationship between post-occlusive reactive hyperemia and the level of training and lifestyle in young adults.European journal of applied physiology · 2026Article
- Optimizing In Vivo Perfusion Assessment by Laser Doppler Flowmetry-Effects of Probe Geometry and Signal Normalization.Diagnostics (Basel, Switzerland) · 2026Article
- Comprehensive validation of a compact laser speckle contrast imaging system for vascular function assessment: from the laboratory to the clinic.Medical & biological engineering & computing · 2025Article
- Noninvasive Bedside Approaches for Assessing Microvascular Dysfunction.Journal of vascular research · 2025Review
- The reproducibility of protocols used to mediate a current-induced vasodilation in the human cutaneous microcirculation.PloS one · 2024Article
- Comparison of laser speckle contrast imaging with laser Doppler perfusion imaging for tissue perfusion measurement.Microcirculation (New York, N.Y. : 1994) · 2023Article
- A Multicenter, Investigator-Blinded, Randomized Controlled Trial to Assess the Efficacy of Calf Neuromuscular Electrical Stimulation Program on Walking Performance in Peripheral Artery Disease: The ELECTRO-PAD Study Protocol.Journal of clinical medicine · 2022Article
- Multiple Laser Doppler Flowmetry Probes Increase the Reproducibility of Skin Blood Flow Measurements.Frontiers in physiology · 2022Article
- Commentary: Multiple laser doppler flowmetry probes increase the reproducibility of skin blood flow measurements.Frontiers in physiology · 2022Article
- Skin Microhemodynamics and Mechanisms of Its Regulation in Type 2 Diabetes Mellitus.Biophysics · 2022Article
- Increased blood pressure and impaired endothelial function after accelerated growth in IVF/ICSI children.Human reproduction open · 2020Article
- Article
- Effects of a short residential thermal spa program to prevent work-related stress/burnout on stress biomarkers: the ThermStress proof of concept study.The Journal of international medical research · 2019Article
- Objectively Measured Aerobic Fitness is Not Related to Vascular Health Outcomes and Cardiovascular Disease Risk In 9-10 Year Old Children.Journal of sports science & medicine · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEndothelial dysfunction precedes atherosclerosis. Vasodilation induced by acetylcholine (ACh) is a specific test of endothelial function. Reproducibility of laser techniques such as laser-Doppler-flowmetry (LDF) and Laser-speckle-contrast-imaging (LSCI) to detect ACh vasodilation is debated and results expressions lack standardization. We aimed to study at a 7-day interval (i) the inter-subject reproducibility, (ii) the intra-subjects reproducibility, and (iii) the effect of the results expressions over variability. METHODS AND
resultsUsing LDF and LSCI simultaneously, we performed two different ACh-iontophoresis protocols. The maximal ACh vasodilation (peak-ACh) was expressed as absolute or normalized flow or conductance values. Inter-subject reproducibility was expressed as coefficient of variation (inter-CV,%). Intra-subject reproducibility was expressed as within subject coefficients of variation (intra-CV,%), and intra-class correlation coefficients (ICC). Fifteen healthy subjects were included. The inter-subject reproducibility of peak-ACh depended upon the expression of the results and ranged from 55% to 162% for LDF and from 17% to 83% for LSCI. The intra-subject reproducibility (intra-CV/ICC) of peak-ACh was reduced when assessed with LSCI compared to LDF no matter how the results were expressed and whatever the protocol used. The highest intra-subject reproducibility was found using LSCI. It was 18.7%/0.87 for a single current stimulation (expressed as cutaneous vascular conductance) and 11.4%/0.61 for multiple current stimulations (expressed as absolute value).
conclusionACh-iontophoresis coupled with LSCI is a promising test to assess endothelial function because it is reproducible, safe, and non-invasive. N°: NCT01664572.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.