ArticleNucleic acids research2013
Reconstitution of hemisomes on budding yeast centromeric DNA.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Cryo-EM structures of CENP-A nucleosomes isolated from human cells.Communications biology · 2026Article
- Comparative modes of chromatin engagement by PAX::FOXO1 fusions in rhabdomyosarcoma.bioRxiv : the preprint server for biology · 2026Article
- Urine cf-nucleosomes: A non-invasive window into human physiology and disease.Cell genomics · 2025Article
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.Molecular cell · 2022Article
- Viral histones: pickpocket's prize or primordial progenitor?Epigenetics & chromatin · 2022Review
- Structural features of nucleosomes in interphase and metaphase chromosomes.Molecular cell · 2021Article
- Centromeres under Pressure: Evolutionary Innovation in Conflict with Conserved Function.Genes · 2020Review
- The Oligomerization Landscape of Histones.Biophysical journal · 2019Article
- Novel genetic tools for probing individual H3 molecules in each nucleosome.Current genetics · 2019Review
- An asymmetric centromeric nucleosome.eLife · 2018Article
- Understanding nucleosome dynamics and their links to gene expression and DNA replication.Nature reviews. Molecular cell biology · 2017Review
- Chromatin dynamics during the cell cycle at centromeres.Nature reviews. Genetics · 2017Review
- Article
- Promiscuous Histone Mis-Assembly Is Actively Prevented by Chaperones.Journal of the American Chemical Society · 2016Article
- Nucleosome dynamics during chromatin remodeling in vivo.Nucleus (Austin, Tex.) · 2016Article
- Shearing of the CENP-A dimerization interface mediates plasticity in the octameric centromeric nucleosome.Scientific reports · 2015Article
- Review
- Review
- The Genetic Map Enters Its Second Century.Genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
The structure of nucleosomes that contain the cenH3 histone variant has been controversial. In budding yeast, a single right-handed cenH3/H4/H2A/H2B tetramer wraps the ∼80-bp Centromere DNA Element II (CDE II) sequence of each centromere into a 'hemisome'. However, attempts to reconstitute cenH3 particles in vitro have yielded exclusively 'octasomes', which are observed in vivo on chromosome arms only when Cse4 (yeast cenH3) is overproduced. Here, we show that Cse4 octamers remain intact under conditions of low salt and urea that dissociate H3 octamers. However, particles consisting of two DNA duplexes wrapped around a Cse4 octamer and separated by a gap efficiently split into hemisomes. Hemisome dimensions were confirmed using a calibrated gel-shift assay and atomic force microscopy, and their identity as tightly wrapped particles was demonstrated by gelFRET. Surprisingly, Cse4 hemisomes were stable in 4 M urea. Stable Cse4 hemisomes could be reconstituted using either full-length or tailless histones and with a 78-bp CDEII segment, which is predicted to be exceptionally stiff. We propose that CDEII DNA stiffness evolved to favor Cse4 hemisome over octasome formation. The precise correspondence between Cse4 hemisomes resident on CDEII in vivo and reconstituted on CDEII in vitro without any other factors implies that CDEII is sufficient for hemisome assembly.
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