ArticleNucleic acids research2013
De novo piRNA cluster formation in the Drosophila germ line triggered by transgenes containing a transcribed transposon fragment.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 80 citations in OpenAlex.
- Adaptive P-element Insertions in a Long Non-Coding RNA are Potential Emerging piRNA Clusters.Genome biology and evolution · 2026Article
- Beyond silencing: integrative multi-omics and spatial profiling unravel the systems-level role of piRNAs in HBV-driven Hepatocarcinogenesis.Molecular biology reports · 2026Review
- Co-evolving infectivity and expression patterns drive the diversification of endogenous retroviruses.The EMBO journal · 2026Article
- Rapid evolution and comparative analysis of piRNA clusters inbioRxiv : the preprint server for biology · 2026Article
- Analysis of somatic piRNAs in the malaria mosquitoRNA biology · 2025Article
- RNA-binding protein Ars2 mediates transcriptional silencing of telomeric repeats and transposable elements in the Drosophila germline.Nucleic acids research · 2025Article
- Article
- Rapid evolution of piRNA clusters in theGenome research · 2024Article
- Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity.BMC biology · 2023Article
- The histone demethylase Kdm3 prevents auto-immune piRNAs production inScience advances · 2023Article
- Off-target piRNA gene silencing in Drosophila melanogaster rescued by a transposable element insertion.PLoS genetics · 2023Article
- The piRNA cluster torimochi is an expanding transposon in cultured silkworm cells.PLoS genetics · 2023Article
- Review
- Article
- Patterns of piRNA Regulation in Drosophila Revealed through Transposable Element Clade Inference.Molecular biology and evolution · 2022Article
- Article
- Viral infection impacts transposable element transcript amounts inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- The evolutionary arms race between transposable elements and piRNAs in Drosophila melanogaster.BMC evolutionary biology · 2020Article
- Nuclear Ccr4-Not mediates the degradation of telomeric and transposon transcripts at chromatin in the Drosophila germline.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PIWI-interacting RNAs (piRNAs) provide defence against transposable element (TE) expansion in the germ line of metazoans. piRNAs are processed from the transcripts encoded by specialized heterochromatic clusters enriched in damaged copies of transposons. How these regions are recognized as a source of piRNAs is still elusive. The aim of this study is to determine how transgenes that contain a fragment of the Long Interspersed Nuclear Elements (LINE)-like I transposon lead to an acquired TE resistance in Drosophila. We show that such transgenes, being inserted in unique euchromatic regions that normally do not produce small RNAs, become de novo bidirectional piRNA clusters that silence I-element activity in the germ line. Strikingly, small RNAs of both polarities are generated from the entire transgene and flanking genomic sequences--not only from the transposon fragment. Chromatin immunoprecipitation analysis shows that in ovaries, the trimethylated histone 3 lysine 9 (H3K9me3) mark associates with transgenes producing piRNAs. We show that transgene-derived hsp70 piRNAs stimulate in trans cleavage of cognate endogenous transcripts with subsequent processing of the non-homologous parts of these transcripts into piRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.