ArticlePathogens and disease2013
Transformation of a plasmid-free, genital tract isolate of Chlamydia trachomatis with a plasmid vector carrying a deletion in CDS6 revealed that this gene regulates inclusion phenotype.
Article in Pathogens and disease, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- A new core-genome MLST approach for Chlamydia trachomatis genomic surveillance and research.Nature communications · 2026Article
- Development of shuttle vector-based transformation systems for veterinary and zoonotic chlamydiae.Microbiology spectrum · 2025Article
- Zoonotic and other veterinary chlamydiae - an update, the role of the plasmid and plasmid-mediated transformation.Pathogens and disease · 2024Review
- Whole Genome Sequencing of aCurrent issues in molecular biology · 2023Article
- The Nature and Extent of Plasmid Variation inMicroorganisms · 2020Article
- Modulation of Host Cell Metabolism byMicrobiology spectrum · 2019Review
- The Genetic Transformation of Chlamydia pneumoniae.mSphere · 2018Article
- Population-based analysis of ocular Chlamydia trachomatis in trachoma-endemic West African communities identifies genomic markers of disease severity.Genome medicine · 2018Article
- Genetic Transformation of aFrontiers in cellular and infection microbiology · 2018Article
- TheWellcome open research · 2018Article
- Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.Microbiology and molecular biology reviews : MMBR · 2016Review
- Intrauterine infection with plasmid-free Chlamydia muridarum reveals a critical role of the plasmid in chlamydial ascension and establishes a model for evaluating plasmid-independent pathogenicity.Infection and immunity · 2015Article
- Plasmid-Encoded Pgp5 Is a Significant Contributor to Chlamydia muridarum Induction of Hydrosalpinx.PloS one · 2015Article
- Trachoma and Ocular Chlamydial Infection in the Era of Genomics.Mediators of inflammation · 2015Review
- Plasmid-encoded Pgp3 is a major virulence factor for Chlamydia muridarum to induce hydrosalpinx in mice.Infection and immunity · 2014Article
- Reply to letter to the editor RE: "in vivo whole animal body imaging reveals colonization of Chlamydia muridarum to the lower genital tract at early stages of infection".Molecular imaging and biology · 2014Article
- In vivo whole animal body imaging reveals colonization of Chlamydia muridarum to the lower genital tract at early stages of infection.Molecular imaging and biology · 2014Article
- The genetic basis of plasmid tropism between Chlamydia trachomatis and Chlamydia muridarum.Pathogens and disease · 2014Article
- Plasmid CDS5 influences infectivity and virulence in a mouse model of Chlamydia trachomatis urogenital infection.Infection and immunity · 2014Article
- Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities.FEMS microbiology reviews · 2014Review
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6 authors.
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Abstract
The development of a plasmid-based genetic transformation protocol for Chlamydia trachomatis provides the basis for the detailed investigation of the function of the chlamydial plasmid and its individual genes or coding sequences (CDS). In this study we constructed a plasmid vector with CDS6 deleted (pCDS6KO) from the original Escherichia coli/C. trachomatis shuttle vector pGFP::SW2. pCDS6KO was transformed into a clinical isolate of C. trachomatis from Sweden that is plasmid-free (C. trachomatis SWFP-). Penicillin-resistant transformants expressing the green fluorescent protein were selected. These transformants did not stain with iodine, indicating that this property is regulated by CDS6 or its gene product. In addition, mature inclusions of C. trachomatis SWFP- transformed by pCDS6KO displayed an identical morphological phenotype to the untransformed plasmid-free recipient host. In this phenotype the morphology of inclusions was altered with the chlamydiae lining the periphery of the inclusion leaving a 'hole' in the centre. These green fluorescent inclusions appear 'doughnut-shaped' with an empty centre when examined under blue light, giving rise to a characteristic 'black hole' phenotype. Our study demonstrates the power of the new genetic system for investigating chlamydial gene function using gene deletion technology.
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