ArticleClinical oral investigations2014
In vitro characterization of a synthetic calcium phosphate bone graft on periodontal ligament cell and osteoblast behavior and its combination with an enamel matrix derivative.
Article in Clinical oral investigations, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Combination of Enamel Matrix Derivatives with Bone Graft vs Bone Graft Alone in the Treatment of Periodontal Intrabony and Furcation Defects: A Systematic Review and Meta-Analysis.Oral health & preventive dentistry · 2024Pooled it
- Evaluation of autogenous tooth bone graft's impact on cellular behaviour and mineralized tissue formation: an in vitro study.BMC oral health · 2026Article
- Cellular Behaviors of Periodontal Ligament Stem Cells in the Presence of Bone Grafting Biomaterials, In-Vitro Study.Life (Basel, Switzerland) · 2022Article
- Comparison of Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells through Application of Two β-tricalcium Phosphate Products: AnJournal of dentistry (Shiraz, Iran) · 2022Article
- Differentiation of Bone Marrow Stem Cells in Presence of Human and Xenogeneic Acellular Dermal Matrix and Collagen Membrane: anJournal of dentistry (Shiraz, Iran) · 2022Article
- Combination of enamel matrix derivative and hyaluronic acid inhibits lipopolysaccharide-induced inflammatory response on human epithelial and bone cells.Clinical oral investigations · 2022Article
- Effect of enamel matrix derivative liquid combined with synthetic bone substitute on bone regeneration in a rabbit calvarial model.Clinical oral investigations · 2021Article
- Recombinant human bone morphogenetic protein (rhBMP)9 induces osteoblast differentiation when combined with demineralized freeze-dried bone allografts (DFDBAs) or biphasic calcium phosphate (BCP).Clinical oral investigations · 2017Article
- Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material.Clinical oral investigations · 2016Article
- Osteoinductive potential of 4 commonly employed bone grafts.Clinical oral investigations · 2016Article
- Enamel matrix derivative improves gingival fibroblast cell behavior cultured on titanium surfaces.Clinical oral investigations · 2016Article
- Effect of bone graft density on in vitro cell behavior with enamel matrix derivative.Clinical oral investigations · 2015Article
- Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds.Journal of tissue engineering · 2015Article
- Influence of enamel matrix derivative on cells at different maturation stages of differentiation.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesRecent studies suggest that a combination of enamel matrix derivative (EMD) with grafting material may improve periodontal wound healing/regeneration. Newly developed calcium phosphate (CaP) ceramics have been demonstrated a viable synthetic replacement option for bone grafting filler materials.
aimsThis study aims to test the ability for EMD to adsorb to the surface of CaP particles and to determine the effect of EMD on downstream cellular pathways such as adhesion, proliferation, and differentiation of primary human osteoblasts and periodontal ligament (PDL) cells. MATERIALS AND
methodsEMD was adsorbed onto CaP particles and analyzed for protein adsorption patterns via scanning electron microscopy and high-resolution immunocytochemistry with an anti-EMD antibody. Cell attachment and cell proliferation were quantified using CellTiter 96 One Solution Cell Assay (MTS). Cell differentiation was analyzed using real-time PCR for genes encoding Runx2, alkaline phosphatase, osteocalcin, and collagen1α1, and mineralization was assessed using alizarin red staining.
resultsAnalysis of cell attachment revealed significantly higher number of cells attached to EMD-adsorbed CaP particles when compared to control and blood-adsorbed samples. EMD also significantly increased cell proliferation at 3 and 5 days post-seeding. Moreover, there were significantly higher mRNA levels of osteoblast differentiation markers including collagen1α1, alkaline phosphatase, and osteocalcin in osteoblasts and PDL cells cultured on EMD-adsorbed CaP particles at various time points.
conclusionThe present study suggests that the addition of EMD to CaP grafting particles may influence periodontal regeneration by stimulating PDL cell and osteoblast attachment, proliferation, and differentiation. Future in vivo and clinical studies are required to confirm these findings. CLINICAL RELEVANCE: The combination of EMD and CaP may represent an option for regenerative periodontal therapy in advanced intrabony defects.
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