ArticleBMC research notes2013
Long term culture of MDCK strains alters chromosome content.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- From single-cell cloning to high-yield influenza virus production - implementing advanced technologies in vaccine process development.Engineering in life sciences · 2024Article
- Characterization and Validation of Canine P-Glycoprotein-Deficient MDCK II Cell Lines for Efflux Substrate Screening.Pharmaceutical research · 2020Article
- Article
- Tubuloids derived from human adult kidney and urine for personalized disease modeling.Nature biotechnology · 2019Article
- A novel canine kidney cell line model for the evaluation of neoplastic development: karyotype evolution associated with spontaneous immortalization and tumorigenicity.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells.BMC research notes · 2015Article
- 2D and 3D chromosome painting in malaria mosquitoes.Journal of visualized experiments : JoVE · 2014Article
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Abstract
backgroundThe Madin-Darby Canine Kidney (MDCK) cell line and its different strains are widely used as models for studying epithelial simple polarity. Recently Dukes et al. [BMC Cell Biology 12:43, 2011] provided a useful guide to the different MDCK strains, with a directory of where to buy them. The present work focused on chromosome content of MDCK cells, a parameter often disregarded by researchers working with these cells.
findingsUsing a general and reliable method for obtaining high yield of metaphasic preparations, the chromosome content of MDCK, MDCK I, MDCK II obtained from reliable sources was determined after maintaining them in culture for various periods of time. Within two months significant changes were observed in the range and the mean number of chromosomes of MDCK I and MDCK II cells. MDCK II cells routinely cultured in six different laboratories were also examined. In some of these cultures the cells have considerably drifted as shown by a high scattering in their number of chromosomes.
conclusionThese results entitle me to encourage researchers using MDCK cells obtained from reliable sources, to determine their chromosomal content upon receipt, to check this content after several passages, to use this feature to follow the possible drift of these cells, and finally to avoid working with cells maintained for more than two months in culture.
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