ArticleBiomacromolecules2013
Structural characterization of a model gram-negative bacterial surface using lipopolysaccharides from rough strains of Escherichia coli.
Article in Biomacromolecules, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 96 citations in OpenAlex.
- Lipopolysaccharide and Coagulation Factor XII: Biophysics of Contact Activation in Infection.Seminars in thrombosis and hemostasis · 2026Review
- Discriminating Between Living and Dead Bacteria With a Field-Effect Transistor Based on Nano-Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Lipopolysaccharide insertion and vesicle mediated turnover drive growth independent outer membrane adaptation in Escherichia coli.Nature communications · 2026Article
- Random Forest Modeling to Predict Small Molecule Accumulation in Gram-Negative Bacteria.ACS infectious diseases · 2026Article
- Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods.European biophysics journal : EBJ · 2026Article
- Article
- Factors Promoting Lipopolysaccharide Uptake by Synthetic Lipid Droplets.bioRxiv : the preprint server for biology · 2024Article
- Low salinity stress increases the risk of Vibrio parahaemolyticus infection and gut microbiota dysbiosis in Pacific white shrimp.BMC microbiology · 2024Article
- Antibiotics and hexagonal order in the bacterial outer membrane.Nature communications · 2023Article
- Review
- Antibiotic polymyxin arranges lipopolysaccharide into crystalline structures to solidify the bacterial membrane.Nature communications · 2022Article
- Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials.Membranes · 2022Review
- Exploiting neutron scattering contrast variation in biological membrane studies.Biophysics reviews · 2022Review
- Effect of pH-Regulation on the Capture of Lipopolysaccharides fromMaterials (Basel, Switzerland) · 2021Article
- Studying the surfaces of bacteria using neutron scattering: finding new openings for antibiotics.Biochemical Society transactions · 2020Review
- Structure and Conformation of Wild-Type Bacterial Lipopolysaccharide Layers at Air-Water Interfaces.Biophysical journal · 2019Article
- Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect.mBio · 2019Article
- Distribution of mechanical stress in the Escherichia coli cell envelope.Biochimica et biophysica acta. Biomembranes · 2018Article
- Liquid crystalline bacterial outer membranes are critical for antibiotic susceptibility.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Conformation of Single and Interacting Lipopolysaccharide Surfaces Bearing O-Side Chains.Biophysical journal · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Lipopolysaccharides (LPS) make up approximately 75% of the Gram-negative bacterial outer membrane (OM) surface, but because of the complexity of the molecule, there are very few model OMs that include LPS. The LPS molecule consists of lipid A, which anchors the LPS within the OM, a core polysaccharide region, and a variable O-antigen polysaccharide chain. In this work we used RcLPS (consisting of lipid A plus the first seven sugars of the core polysaccharide) from a rough strain of Escherichia coli to form stable monolayers of LPS at the air-liquid interface. The vertical structure RcLPS monolayers were characterized using neutron and X-ray reflectometry, while the lateral structure was investigated using grazing incidence X-ray diffraction and Brewster angle microscopy. It was found that RcLPS monolayers at surface pressures of 20 mN m(-1) and above are resolved as hydrocarbon tails, an inner headgroup, and an outer headgroup of polysaccharide with increasing solvation from tails to outer headgroups. The lateral organization of the hydrocarbon lipid chains displays an oblique hexagonal unit cell at all surface pressures, with only the chain tilt angle changing with surface pressure. This is in contrast to lipid A, which displays hexagonal or, above 20 mN m(-1), distorted hexagonal packing. This work provides the first complete structural analysis of a realistic E. coli OM surface model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.