Evidence map›Paper›PMID 23616566›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013

Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow.

Shankar J Evani, Rajesh G Prabhu, V Gnanaruban, Ender A Finol, Anand K Ramasubramanian

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PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed, 2 pooled it
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 2 syntheses or guidelines pooled it, 89 citations in OpenAlex.

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5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shankar J EvaniDepartment of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Rajesh G Prabhu
V Gnanaruban
Ender A Finol
Anand K Ramasubramanian
The University of Texas at San Antonio · US

Funding

Role of fluid flow on Chlamydia pneumoniae-exacerbated atherosclerosisSC1HL112629 · NHLBI · UNIVERSITY OF TEXAS SAN ANTONIO · PI RAMASUBRAMANIAN, ANAND K · 2011 to 2015
$1.8M
NHLBI NIH HHS HL112629NHLBI NIH HHS SC1 HL112629
6 · The paper itself

Abstract

Endothelial adhesion is necessary for the hematogenous dissemination of tumor cells. However, the metastatic breast tumor cell MDA-MB-231 does not bind to the endothelium under physiological flow conditions, suggesting alternate mechanisms of adhesion. Since monocytes are highly represented in the tumor microenvironment, and also bind to endothelium during inflammation, we hypothesized that the monocytes assist in the arrest of MDA-MB-231 on the endothelium. Using in vitro models of the dynamic shear environment of the vasculature, we show that TNF-α-activated THP1/primary human monocytes and MDA-MB-231 cells form stable aggregates, and that the monocytes in these aggregates mediate the adhesion of otherwise nonadherent MDA-MB-231 cells to inflamed endothelium under flow (55±2.4 vs. 1.7±0.82 at a shear stress of 0.5 dyn/cm(2), P<0.01). We also show that the hydrodynamic forces determine the size and orientation of aggregates adhered to the endothelium, and strongly favor the attachment of small aggregates with tumor cells downstream of flow (74-86% doublets at 0.5-2 dyn/cm(2), P<0.01). The 5-fold up-regulation of ICAM-1 on TNF-α-activated MDA-MB-231 cells through the Nf-κB pathway was found to be critical in MDA-MB-231-monocyte aggregation and endothelial adhesion. Our results demonstrate that, under inflammatory conditions, monocytes may serve to disseminate tumor cells through circulation, and the tumor-monocyte-endothelial axis may represent a new therapeutic target to reduce cancer metastasis.

Indexed as

Breast NeoplasmsCell AdhesionCell AggregationCell Line, TumorCells, CulturedComputer SimulationEndothelium, VascularHumansIntercellular Adhesion Molecule-1KineticsMonocytesNeoplasm MetastasisNF-kappa BSignal TransductionStress, MechanicalTumor Necrosis Factor-alphaIntercellular Adhesion Molecule-1NF-kappa BTumor Necrosis Factor-alphaaggregationMDA-MB-231shear

Identifiers

PMID23616566
PMCPMC3714575
OpenAlexW2095976536

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.