ArticleThe Journal of biological chemistry2013
Role of central metabolism in the osmoadaptation of the halophilic bacterium Chromohalobacter salexigens.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 63 citations in OpenAlex.
- Limits of bacterial osmoadaptation during planktonic and biofilm growth: a step toward effective biofouling control.Applied and environmental microbiology · 2026Article
- Adaptability ofCombinatorial chemistry & high throughput screening · 2026Article
- Towards economic and sustainable production of poly-3-hydroxybutyrate byFrontiers in microbiology · 2026Article
- Article
- Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation.Applied microbiology and biotechnology · 2024Article
- Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights.Microbial cell factories · 2024Article
- Co-Production of Poly(3-hydroxybutyrate) and Gluconic Acid from Glucose byBioengineering (Basel, Switzerland) · 2023Article
- Whole-genome sequencing and genome-scale metabolic modeling of Chromohalobacter canadensis 85B to explore its salt tolerance and biotechnological use.MicrobiologyOpen · 2022Article
- Mineralogy of Bioprecipitate Evolution over Induction Times Mediated by Halophilic Bacteria under Various Mg/Ca Molar Ratios.ACS omega · 2022Article
- Histidinibacterium aquaticum sp. nov., Isolated from Salt-Field Sea Water.Current microbiology · 2022Article
- Red algae industrial residues as a sustainable carbon platform for the co-production of poly-3-hydroxybutyrate and gluconic acid byFrontiers in bioengineering and biotechnology · 2022Article
- Salt-stress adaptation of yeast as a simple method to improve high-gravity fermentation in an industrial medium.Applied microbiology and biotechnology · 2021Article
- Genome analysis of the salt-resistant Paludifilum halophilum DSM 102817World journal of microbiology & biotechnology · 2021Article
- New insights into hydroxyectoine synthesis and its transcriptional regulation in the broad-salt growing halophilic bacterium Chromohalobacter salexigens.Microbial biotechnology · 2021Article
- The organosulfur compound dimethylsulfoniopropionate (DMSP) is utilized as an osmoprotectant byApplied and environmental microbiology · 2021Article
- CosR Is a Global Regulator of the Osmotic Stress Response with Widespread Distribution among Bacteria.Applied and environmental microbiology · 2020Article
- Anaplerotic Pathways inFrontiers in microbiology · 2020Article
- High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium.Microbial cell factories · 2019Article
- Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden-Meyerhof-Parnas and Entner-Doudoroff pathways.Microbial cell factories · 2019Article
- Protective effects of compatible solute ectoine against ethanol-induced toxic alterations in Daphnia magna.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial osmoadaptation involves the cytoplasmic accumulation of compatible solutes to counteract extracellular osmolarity. The halophilic and highly halotolerant bacterium Chromohalobacter salexigens is able to grow up to 3 m NaCl in a minimal medium due to the de novo synthesis of ectoines. This is an osmoregulated pathway that burdens central metabolic routes by quantitatively drawing off TCA cycle intermediaries. Consequently, metabolism in C. salexigens has adapted to support this biosynthetic route. Metabolism of C. salexigens is more efficient at high salinity than at low salinity, as reflected by lower glucose consumption, lower metabolite overflow, and higher biomass yield. At low salinity, by-products (mainly gluconate, pyruvate, and acetate) accumulate extracellularly. Using [1-(13)C]-, [2-(13)C]-, [6-(13)C]-, and [U-(13)C6]glucose as carbon sources, we were able to determine the main central metabolic pathways involved in ectoines biosynthesis from glucose. C. salexigens uses the Entner-Doudoroff pathway rather than the standard glycolytic pathway for glucose catabolism, and anaplerotic activity is high to replenish the TCA cycle with the intermediaries withdrawn for ectoines biosynthesis. Metabolic flux ratios at low and high salinity were similar, revealing a certain metabolic rigidity, probably due to its specialization to support high biosynthetic fluxes and partially explaining why metabolic yields are so highly affected by salinity. This work represents an important contribution to the elucidation of specific metabolic adaptations in compatible solute-accumulating halophilic bacteria.
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