ArticlePloS one2013
UGT74D1 is a novel auxin glycosyltransferase from Arabidopsis thaliana.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 44 papers.
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44 citing papers in PubMed, 83 citations in OpenAlex.
- Metabolism meets transport: a new perspective on auxin homeostasis at subcellular level.Planta · 2026Review
- GH3 phylogenetic subgroups define divergent routes of auxin inactivation via aspartate and glutamine conjugation.Plant physiology · 2026Article
- PgUGT84K2, a Panax ginseng glycosyltransferase, catalyses IBA glycosylation and enhances abiotic stress tolerance in transgenic Arabidopsis.Plant biology (Stuttgart, Germany) · 2026Article
- Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.Rice (New York, N.Y.) · 2026Article
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- Identification of Auxin, Cytokinin, Transcription Factors, and Other Zygotic Embryogenesis-Related Genes inPlants (Basel, Switzerland) · 2025Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- AtIAR1 is a Zn transporter that regulates auxin metabolism in Arabidopsis thaliana.Journal of experimental botany · 2024Article
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Al-induced CsUGT84J2 enhances flavonol and auxin accumulation to promote root growth in tea plants.Horticulture research · 2023Article
- Regulation of Salicylic Acid and N-Hydroxy-Pipecolic Acid in Systemic Acquired Resistance.The plant pathology journal · 2023Review
- PIF7 is a master regulator of thermomorphogenesis in shade.Nature communications · 2022Article
- Transcriptome Analysis and Gene Expression Profiling of the Peanut Small Seed Mutant Identified Genes Involved in Seed Size Control.International journal of molecular sciences · 2022Article
- Article
- Differences in the Abundance of Auxin Homeostasis Proteins Suggest Their Central Roles for In Vitro Tissue Differentiation inPlants (Basel, Switzerland) · 2021Article
- Identification of potential pathways associated with indole-3-butyric acid in citrus bud germination via transcriptomic analysis.Functional & integrative genomics · 2021Article
- Auxin Metabolome Profiling in the Arabidopsis Endoplasmic Reticulum Using an Optimised Organelle Isolation Protocol.International journal of molecular sciences · 2021Article
- Specific decorations of 17-hydroxygeranyllinalool diterpene glycosides solve the autotoxicity problem of chemical defense in Nicotiana attenuata.The Plant cell · 2021Article
- On the Evolutionary Origins of Land Plant Auxin Biology.Cold Spring Harbor perspectives in biology · 2021Review
- Biochemical Characterization of Recombinant UDPG-Dependent IAA Glucosyltransferase from Maize (International journal of molecular sciences · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxin is one type of phytohormones that plays important roles in nearly all aspects of plant growth and developmental processes. The glycosylation of auxins is considered to be an essential mechanism to control the level of active auxins. Thus, the identification of auxin glycosyltransferases is of great significance for further understanding the auxin regulation. In this study, we biochemically screened the group L of Arabidopsis thaliana glycosyltransferase superfamily for enzymatic activity toward auxins. UGT74D1 was identified to be a novel auxin glycosyltransferase. Through HPLC and LC-MS analysis of reaction products in vitro by testing eight substrates including auxins and other compounds, we found that UGT74D1 had a strong glucosylating activity toward indole-3-butyric acid [IBA], indole-3-propionic acid [IPA], indole-3-acetic acid [IAA] and naphthaleneacetic acid [NAA], catalyzing them to form corresponding glucose esters. Biochemical characterization showed that this enzyme had a maximum activity in HEPES buffer at pH 6.0 and 37°C. In addition, the enzymatic activity analysis of crude protein and the IBA metabolite analysis from transgenic Arabidopsis plants overexpressing UGT74D1 gene were also carried out. Experimental results indicated that over-production of the UGT74D1 in plants indeed led to increased level of the glucose conjugate of IBA. Moreover, UGT74D1 overexpression lines displayed curling leaf phenotype, suggesting a physiological role of UGT74D1 in affecting the activity of auxins. Our current data provide a new target gene for further genetic studies to understand the auxin regulation by glycosylation in plants.
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