ArticlePloS one2013
RNAi knock-down of LHCBM1, 2 and 3 increases photosynthetic H2 production efficiency of the green alga Chlamydomonas reinhardtii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 115 citations in OpenAlex.
- Co-Cultivation With New Glucose-Sparing Chlorella Algae Boosts Tissue Culture Efficiency by Reducing Cell Waste.Biotechnology journal · 2025Article
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- Simultaneous CAS9 editing of cpPlant direct · 2023Article
- Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production.Plant physiology · 2022Article
- Techno-economic evaluation of microalgae high-density liquid fuel production at 12 international locations.Biotechnology for biofuels · 2021Article
- Review
- Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms.Plant physiology · 2019Article
- Biomass from microalgae: the potential of domestication towards sustainable biofactories.Microbial cell factories · 2018Review
- High-throughput optimisation of light-driven microalgae biotechnologies.Scientific reports · 2018Article
- Unrestrained markerless trait stacking inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris.Scientific reports · 2017Article
- Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green AlgaFrontiers in plant science · 2017Article
- The function of LHCBM4/6/8 antenna proteins in Chlamydomonas reinhardtii.Journal of experimental botany · 2017Article
- Challenges and opportunities for hydrogen production from microalgae.Plant biotechnology journal · 2016Review
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- High-Resolution Profiling of a Synchronized Diurnal Transcriptome from Chlamydomonas reinhardtii Reveals Continuous Cell and Metabolic Differentiation.The Plant cell · 2015Article
- Implementation of photobiological H2 production: the O 2 sensitivity of hydrogenases.Photosynthesis research · 2015Article
- Cell Surface and Membrane Engineering: Emerging Technologies and Applications.Journal of functional biomaterials · 2015Review
- Engineering photosynthetic organisms for the production of biohydrogen.Photosynthesis research · 2015Review
- Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single cell green algae (microalgae) are rapidly emerging as a platform for the production of sustainable fuels. Solar-driven H2 production from H2O theoretically provides the highest-efficiency route to fuel production in microalgae. This is because the H2-producing hydrogenase (HYDA) is directly coupled to the photosynthetic electron transport chain, thereby eliminating downstream energetic losses associated with the synthesis of carbohydrate and oils (feedstocks for methane, ethanol and oil-based fuels). Here we report the simultaneous knock-down of three light-harvesting complex proteins (LHCMB1, 2 and 3) in the high H2-producing Chlamydomonas reinhardtii mutant Stm6Glc4 using an RNAi triple knock-down strategy. The resultant Stm6Glc4L01 mutant exhibited a light green phenotype, reduced expression of LHCBM1 (20.6% ±0.27%), LHCBM2 (81.2% ±0.037%) and LHCBM3 (41.4% ±0.05%) compared to 100% control levels, and improved light to H2 (180%) and biomass (165%) conversion efficiencies. The improved H2 production efficiency was achieved at increased solar flux densities (450 instead of ∼100 µE m(-2) s(-1)) and high cell densities which are best suited for microalgae production as light is ideally the limiting factor. Our data suggests that the overall improved photon-to-H2 conversion efficiency is due to: 1) reduced loss of absorbed energy by non-photochemical quenching (fluorescence and heat losses) near the photobioreactor surface; 2) improved light distribution in the reactor; 3) reduced photoinhibition; 4) early onset of HYDA expression and 5) reduction of O2-induced inhibition of HYDA. The Stm6Glc4L01 phenotype therefore provides important insights for the development of high-efficiency photobiological H2 production systems.
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