ArticlePloS one2013
Gene silencing of BnTT10 family genes causes retarded pigmentation and lignin reduction in the seed coat of Brassica napus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Review
- Article
- Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L.Plant biotechnology journal · 2024Article
- Molecular evolution analysis of MYB5 in Brassicaceae with specific focus on seed coat color of Brassica napus.BMC plant biology · 2024Article
- Unraveling the mechanism of flower color variation inFrontiers in plant science · 2024Article
- Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.Nature communications · 2023Article
- Review
- Genome-wide analysis of the laccase (LAC) gene family inFrontiers in plant science · 2023Article
- Comparative transcriptome analysis identifies candidate genes related to seed coat color in rapeseed.Frontiers in plant science · 2023Article
- The Flavonoid Biosynthesis and Regulation inInternational journal of molecular sciences · 2022Review
- DkmiR397 Regulates Proanthocyanidin Biosynthesis via Negative ModulatingInternational journal of molecular sciences · 2022Article
- Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation ofInternational journal of molecular sciences · 2022Article
- Transcriptome Analysis of MYB Genes and Patterns of Anthocyanin Accumulation During Seed Development in Wheat.Evolutionary bioinformatics online · 2022Article
- The MicroRNA397a-LACCASE17 module regulates lignin biosynthesis inFrontiers in plant science · 2022Article
- Seed-coat protective neolignans are produced by the dirigent protein AtDP1 and the laccase AtLAC5 in Arabidopsis.The Plant cell · 2021Article
- A R2R3-MYB gene-based marker for the non-darkening seed coat trait in pinto and cranberry beans (Phaseolus vulgaris L.) derived from 'Wit-rood boontje'.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- What Do We Know About the Genetic Basis of Seed Desiccation Tolerance and Longevity?International journal of molecular sciences · 2020Review
- Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress inMolecules (Basel, Switzerland) · 2019Article
- Transcriptomic comparison between developing seeds of yellow- and black-seeded Brassica napus reveals that genes influence seed quality.BMC plant biology · 2019Article
- Integrated analysis of mRNA-seq and miRNA-seq in calyx abscission zone of Korla fragrant pear involved in calyx persistence.BMC plant biology · 2019Article
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7 authors.
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Abstract
Yellow-seed (i.e., yellow seed coat) is one of the most important agronomic traits of Brassica plants, which is correlated with seed oil and meal qualities. Previous studies on the Brassicaceae, including Arabidopsis and Brassica species, proposed that the seed-color trait is correlative to flavonoid and lignin biosynthesis, at the molecular level. In Arabidopsis thaliana, the oxidative polymerization of flavonoid and biosynthesis of lignin has been demonstrated to be catalyzed by laccase 15, a functional enzyme encoded by the AtTT10 gene. In this study, eight Brassica TT10 genes (three from B. napus, three from B. rapa and two from B. oleracea) were isolated and their roles in flavonoid oxidation/polymerization and lignin biosynthesis were investigated. Based on our phylogenetic analysis, these genes could be divided into two groups with obvious structural and functional differentiation. Expression studies showed that Brassica TT10 genes are active in developing seeds, but with differential expression patterns in yellow- and black-seeded near-isogenic lines. For functional analyses, three black-seeded B. napus cultivars were chosen for transgenic studies. Transgenic B. napus plants expressing antisense TT10 constructs exhibited retarded pigmentation in the seed coat. Chemical composition analysis revealed increased levels of soluble proanthocyanidins, and decreased extractable lignin in the seed coats of these transgenic plants compared with that of the controls. These findings indicate a role for the Brassica TT10 genes in proanthocyanidin polymerization and lignin biosynthesis, as well as seed coat pigmentation in B. napus.
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