ArticlePloS one2013
Dried blood spot sampling for hepatitis B virus serology and molecular testing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 3 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 3 syntheses or guidelines pooled it, 70 citations in OpenAlex.
- Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review.PloS one · 2021Pooled it
- Diagnostic accuracy of serological diagnosis of hepatitis C and B using dried blood spot samples (DBS): two systematic reviews and meta-analyses.BMC infectious diseases · 2017Pooled it
- Diagnostic accuracy of detection and quantification of HBV-DNA and HCV-RNA using dried blood spot (DBS) samples - a systematic review and meta-analysis.BMC infectious diseases · 2017Pooled it
- Hepatitis B Virus Diagnosis Using Dried Blood Spots in the D.R. Congo: Overcoming Misdiagnosis to Achieve 2030 WHO Targets.Medical sciences (Basel, Switzerland) · 2026Article
- Optimization of dried blood spot for hepatitis B virus surface antibody quantification.PloS one · 2025Article
- A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate.Scientific reports · 2024Article
- OPTIMIZATION OF DRIED BLOOD SPOT ASSAYS FOR HEPATITIS B VIRUS SURFACE ANTIBODY QUANTIFICATION.bioRxiv : the preprint server for biology · 2024Article
- Hepatitis B prevention and treatment needs in women in Senegal (ANRS 12356 AmBASS survey).BMC public health · 2023Article
- Using Dried Blood Spots for a Sero-Surveillance Study of Maternally Derived Antibody against Group B Streptococcus.Vaccines · 2023Article
- Dried blood spot is the feasible matrix for detection of some but not all hepatitis B virus markers of infection.BMC research notes · 2022Article
- Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection.Scientific reports · 2022Article
- Article
- Detection and quantitation of hepatitis B surface antigen (HBsAg) on dried blood spots: a solution for easy access for hepatitis B diagnosis and elimination in remote areas.The Pan African medical journal · 2022Article
- Comparison of four extraction methods for the detection of hepatitis B virus DNA in dried blood spot samples.MicrobiologyOpen · 2021Article
- Evaluation of the efficiency of dried blood spot-based measurement of hepatitis B and hepatitis C virus seromarkers.Scientific reports · 2020Article
- Usefulness of automated assays for detecting hepatitis B and C markers in dried blood spot samples.BMC research notes · 2019Article
- Article
- Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis.Canadian journal of gastroenterology & hepatology · 2019Review
- Using dried blood spot for the detection of HBsAg and anti-HCV antibodies in Cameroon.BMC research notes · 2018Article
- Hepatitis B virus in drug users in France: prevalence and vaccination history, ANRS-Coquelicot Survey 2011-2013.Epidemiology and infection · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND
aimsDried blood spots (DBS) on filter paper have been successfully used to diagnose and monitor several infectious diseases. The aim was to investigate the performance of DBS in hepatitis B virus (HBV) diagnosis using commercial tests in comparison to standard methods.
methodsPaired DBS and plasma samples were collected from 200 patients: 100 patients with HBsAg negative status and 100 patients with HBsAg positive status. In the latter patient, HBeAg reactivity was tested. Ten samples of anti-HBs were collected from people vaccinated against HBV. We also studied 50 patients with positive HBV DNA viral load in plasma and 10 HBV DNA negative patients. HBV genotypes and gene polymerase mutations were determined in 10 randomly selected HBV-infected patients. The DBS sample consisted of 50 µL of whole blood, i.e. a 12-mm paper card.
resultsThe sensitivity thresholds of HBsAg and anti-HBs antibody were 0.30 ± 0.08 IU/mL and 18.11 ± 6.05 IU/mL, respectively, for DBS with 98% sensitivity and 100% specificity. Sensitivity was 98% and specificity 100% for the detection of HBV DNA on a blotter, considering an HBV DNA threshold of 914.1 ± 157.8 IU/ml. Ten patients had an HBeAg positive status in plasma, all were detected positive using DBS. HBV genotyping and mutation detection were successfully performed on DBS, with full concordance between the 10 paired DBS and plasma samples.
conclusionThis study shows DBS is a reliable alternative to plasma specimens for quantifying and detecting HBsAg, anti-HBs, HBeAg and genotyping. DBS may increase the opportunities for HBV testing and treatment follow-up in hard-to-reach individuals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.