ArticlePloS one2013
Modulating gradients in regulatory signals within mesenchymal stem cell seeded hydrogels: a novel strategy to engineer zonal articular cartilage.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Compression injury regulates astrocyte morphology, metabolic function, and extracellular matrix modification in a 3D hydrogel.bioRxiv : the preprint server for biology · 2025Article
- Dynamic Compression Improves Chondrogenesis in the Tissue Engineered Model of Cartilage.Biotechnology and bioengineering · 2025Article
- Advancements in hydrogel design for articular cartilage regeneration: A comprehensive review.Bioactive materials · 2025Review
- Regulation of Oxygen Tension as a Strategy to Control Chondrocytic Phenotype for Cartilage Tissue Engineering and Regeneration.Bioengineering (Basel, Switzerland) · 2024Review
- Mechanotransducive Biomimetic Systems for Chondrogenic Differentiation In Vitro.International journal of molecular sciences · 2021Review
- Measuring and Modeling Oxygen Transport and Consumption in 3D Hydrogels Containing Chondrocytes and Stem Cells of Different Tissue Origins.Frontiers in bioengineering and biotechnology · 2021Article
- Mechanical Stress Inhibits Early Stages of Endogenous Cell Migration: A Pilot Study in an Ex Vivo Osteochondral Model.Polymers · 2020Article
- Effect of interface mechanical discontinuities on scaffold-cartilage integration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Investigating the interplay between substrate stiffness and ligand chemistry in directing mesenchymal stem cell differentiation within 3D macro-porous substrates.Biomaterials · 2018Article
- Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage.NPJ Regenerative medicine · 2018Article
- The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells.Acta biomaterialia · 2017Article
- Recent advances on gradient hydrogels in biomimetic cartilage tissue engineering.F1000Research · 2017Review
- Cyclic Tensile Strain Can Play a Role in Directing both Intramembranous and Endochondral Ossification of Mesenchymal Stem Cells.Frontiers in bioengineering and biotechnology · 2017Article
- Tissue Engineering Whole Bones Through Endochondral Ossification: Regenerating the Distal Phalanx.BioResearch open access · 2015Article
- A developmentally inspired combined mechanical and biochemical signaling approach on zonal lineage commitment of mesenchymal stem cells in articular cartilage regeneration.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Infrapatellar fat pad-derived stem cells maintain their chondrogenic capacity in disease and can be used to engineer cartilaginous grafts of clinically relevant dimensions.Tissue engineering. Part A · 2014Article
- Combining freshly isolated chondroprogenitor cells from the infrapatellar fat pad with a growth factor delivery hydrogel as a putative single stage therapy for articular cartilage repair.Tissue engineering. Part A · 2014Article
- Article
- Engineering large cartilage tissues using dynamic bioreactor culture at defined oxygen conditions.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineering organs and tissues with the spatial composition and organisation of their native equivalents remains a major challenge. One approach to engineer such spatial complexity is to recapitulate the gradients in regulatory signals that during development and maturation are believed to drive spatial changes in stem cell differentiation. Mesenchymal stem cell (MSC) differentiation is known to be influenced by both soluble factors and mechanical cues present in the local microenvironment. The objective of this study was to engineer a cartilaginous tissue with a native zonal composition by modulating both the oxygen tension and mechanical environment thorough the depth of MSC seeded hydrogels. To this end, constructs were radially confined to half their thickness and subjected to dynamic compression (DC). Confinement reduced oxygen levels in the bottom of the construct and with the application of DC, increased strains across the top of the construct. These spatial changes correlated with increased glycosaminoglycan accumulation in the bottom of constructs, increased collagen accumulation in the top of constructs, and a suppression of hypertrophy and calcification throughout the construct. Matrix accumulation increased for higher hydrogel cell seeding densities; with DC further enhancing both glycosaminoglycan accumulation and construct stiffness. The combination of spatial confinement and DC was also found to increase proteoglycan-4 (lubricin) deposition toward the top surface of these tissues. In conclusion, by modulating the environment through the depth of developing constructs, it is possible to suppress MSC endochondral progression and to engineer tissues with zonal gradients mimicking certain aspects of articular cartilage.
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