ArticlePloS one2013
The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells forIranian journal of basic medical sciences · 2026Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- WIN18,446 enhances spermatogonial stem cell homing and fertility after germ cell transplantation by increasing blood-testis barrier permeability.The Journal of reproduction and development · 2023Article
- Recent advances in developing 3D culture systems of spermatogonial stem cell preservation and differentiation: A narrative review.International journal of reproductive biomedicine · 2023Review
- Sperm-carried IGF2 downregulated the expression of mitogens produced by Sertoli cells: A paracrine mechanism for regulating spermatogenesis?Frontiers in endocrinology · 2022Article
- The Regulation of Spermatogonial Stem Cells in an Adult Testis by Glial Cell Line-Derived Neurotrophic Factor.Frontiers in endocrinology · 2022Review
- Spermatogonial stem cell transplantation into nonablated mouse recipient testes.Stem cell reports · 2021Article
- Mammalian germ cell migration during development, growth, and homeostasis.Reproductive medicine and biology · 2019Review
- Aberrant gene expression by Sertoli cells in infertile men with Sertoli cell-only syndrome.PloS one · 2019Article
- Pediatric Germ Cell Tumors: A Developmental Perspective.Advances in urology · 2018Review
- Temporal-Spatial Establishment of Initial Niche for the Primary Spermatogonial Stem Cell Formation Is Determined by an ARID4B Regulatory Network.Stem cells (Dayton, Ohio) · 2017Article
- Protective effects of melatonin and ghrelin on spermatogenesis: A narrative review of the literature.International journal of reproductive biomedicine · 2017Article
- c-Jun Amino-Terminal Kinase is Involved in Valproic Acid-Mediated Neuronal Differentiation of Mouse Embryonic NSCs and Neurite Outgrowth of NSC-Derived Neurons.Neurochemical research · 2017Article
- Male germline stem cells in non-human primates.Primate biology · 2017Review
- Review
- Chemokine (C-X-C) Ligand 12 Facilitates Trafficking of Donor Spermatogonial Stem Cells.Stem cells international · 2016Article
- Wnt signaling pathway participates in valproic acid-induced neuronal differentiation of neural stem cells.International journal of clinical and experimental pathology · 2015Article
- Cell-cycle-dependent colonization of mouse spermatogonial stem cells after transplantation into seminiferous tubules.The Journal of reproduction and development · 2014Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, the biological activity of the stem/progenitor compartment sustains production of mature gametes through spermatogenesis. Spermatogonial stem cells and their progeny belong to the class of undifferentiated spermatogonia, a germ cell population found on the basal membrane of the seminiferous tubules. A large body of evidence has demonstrated that glial cell line-derived neurotrophic factor (GDNF), a Sertoli-derived factor, is essential for in vivo and in vitro stem cell self-renewal. However, the mechanisms underlying this activity are not completely understood. In this study, we show that GDNF induces dose-dependent directional migration of freshly selected undifferentiated spermatogonia, as well as germline stem cells in culture, using a Boyden chamber assay. GDNF-induced migration is dependent on the expression of the GDNF co-receptor GFRA1, as shown by migration assays performed on parental and GFRA1-transduced GC-1 spermatogonial cell lines. We found that the actin regulatory protein vasodilator-stimulated phosphoprotein (VASP) is specifically expressed in undifferentiated spermatogonia. VASP belongs to the ENA/VASP family of proteins implicated in actin-dependent processes, such as fibroblast migration, axon guidance, and cell adhesion. In intact seminiferous tubules and germline stem cell cultures, GDNF treatment up-regulates VASP in a dose-dependent fashion. These data identify a novel role for the niche-derived factor GDNF, and they suggest that GDNF may impinge on the stem/progenitor compartment, affecting the actin cytoskeleton and cell migration.
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