ArticleProceedings of the National Academy of Sciences of the United States of America2013
Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast.Biomolecules · 2023Review
- Developmental roles and molecular mechanisms of Asterix/GTSF1.Wiley interdisciplinary reviews. RNA · 2022Review
- Breakers and amplifiers in chromatin circuitry: acetylation and ubiquitination control the heterochromatin machinery.Current opinion in structural biology · 2021Review
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
- H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly.EMBO reports · 2019Article
- Deciphering the three-domain architecture in schlafens and the structures and roles of human schlafen12 and serpinB12 in transcriptional regulation.Journal of molecular graphics & modelling · 2019Article
- GTSF-1 is required for formation of a functional RNA-dependent RNA Polymerase complex inThe EMBO journal · 2018Article
- Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity.Immunity · 2018Article
- The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts.Genes & development · 2016Article
- Annual meeting of the EpiGeneSys Network of Excellence--Advancing epigenetics towards systems biology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.Genome biology · 2014Article
- Drosophila Gtsf1 is an essential component of the Piwi-mediated transcriptional silencing complex.Genes & development · 2013Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
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Abstract
Noncoding RNAs can modulate gene expression by directing modifications to histones that alter chromatin structure. In fission yeast, siRNAs produced via the RNAi pathway direct modifications associated with heterochromatin formation. siRNAs associate with the RNAi effector protein Argonaute 1 (Ago1), targeting the Ago1-containing RNA-induced transcriptional silencing (RITS) complex to homologous nascent transcripts. This promotes recruitment of the Clr4 complex (CLRC), which mediates methylation of histone H3 on lysine 9 (H3K9me) in cognate chromatin. A key question is how the RNAi and chromatin modification machineries are connected. Stc1 is a small protein recently shown to associate with both Ago1 and CLRC and to play a pivotal role in mediating the RNAi-dependent recruitment of CLRC to chromatin. To understand its mode of action, we have performed a detailed structural and functional analysis of the Stc1 protein. Our analyses reveal that the conserved N-terminal region of Stc1 represents an unusual tandem zinc finger domain, with similarities to common LIM domains but distinguished by a lack of preferred relative orientation of the two zinc fingers. We demonstrate that this tandem zinc finger domain is involved in binding Ago1, whereas the nonconserved C-terminal region mediates association with CLRC. These findings elucidate the molecular basis for the coupling of RNAi to chromatin modification in fission yeast.
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