ArticleProceedings of the National Academy of Sciences of the United States of America2013
Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
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- Technologies for Monoclonal Antibody Discovery and Development.International journal of molecular sciences · 2025Review
- Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.Biomolecules · 2024Article
- High-fidelity large-diversity monoclonal mammalian cell libraries by cell cycle arrested recombinase-mediated cassette exchange.Nucleic acids research · 2023Article
- Article
- Agonist (P1) Antibody Converts Stem Cells into Migrating Beta-Like Cells in Pancreatic Islets.Journal of microbiology and biotechnology · 2022Article
- Neutralizing Antibodies to SARS-CoV-2 Selected from a Human Antibody Library Constructed Decades Ago.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Agonist antibody discovery: Experimental, computational, and rational engineering approaches.Drug discovery today · 2022Review
- Transition Therapy: Tackling the Ecology of Tumor Phenotypic Plasticity.Bulletin of mathematical biology · 2021Article
- Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms.Nature communications · 2021Article
- Antibody Libraries as Tools to Discover Functional Antibodies and Receptor Pleiotropism.International journal of molecular sciences · 2021Review
- Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Selection of a Full Agonist Combinatorial Antibody that Rescues Leptin Deficiency In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Function-based high-throughput screening for antibody antagonists and agonists against G protein-coupled receptors.Communications biology · 2020Article
- Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in Heart.Cells · 2020Article
- Immunity against cancer cells may promote their proliferation and metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- An agonist antibody prefers relapsed AML for induction of cells that kill each other.Scientific reports · 2019Article
- Autocrine-based selection of ligands for personalized CAR-T therapy of lymphoma.Science advances · 2018Article
- Fully human agonist antibodies to TrkB using autocrine cell-based selection from a combinatorial antibody library.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here the generation of antibody agonists from intracellular combinatorial libraries that transdifferentiate human stem cells. Antibodies that are agonists for the granulocyte colony stimulating factor receptor were selected from intracellular libraries on the basis of their ability to activate signaling pathways in reporter cells. We used a specialized "near neighbor" approach in which the entire antibody library and its target receptor are cointegrated into the plasma membranes of a population of reporter cells. This format favors unusual interactions between receptors and their protein ligands and ensures that the antibody acts in an autocrine manner on the cells that produce it. Unlike the natural granulocyte-colony stimulating factor that activates cells to differentiate along a predetermined pathway, the isolated agonist antibodies transdifferentiated human myeloid lineage CD34+ bone marrow cells into neural progenitors. This transdifferentiation by agonist antibodies is different from more commonly used methods because initiation is agenetic. Antibodies that act at the plasma membrane may have therapeutic potential as agents that transdifferentiate autologous cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.