ArticleThe Journal of physiology2013
Ca2+ sensitization pathways accessed by cholinergic neurotransmission in the murine gastric fundus.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 55 citations in OpenAlex.
- Advances in Understanding Calcium Sensitization in Gastrointestinal Smooth Muscles: Pathways, Interstitial Cells, and Disease.Journal of neurogastroenterology and motility · 2026Review
- Fennel Tea Has a Region-Specific Effect on the Motility of the Stomach.Neurogastroenterology and motility · 2025Article
- CaPhysiological reviews · 2024Review
- Altered functional responses by PAR1 agonist in murine dextran sodium sulphate-treated colon.Scientific reports · 2022Article
- Calcium transients in intramuscular interstitial cells of Cajal of the murine gastric fundus and their regulation by neuroeffector transmission.The Journal of physiology · 2022Article
- CaAdvances in experimental medicine and biology · 2022Article
- Contribution of Postjunctional M2 Muscarinic Receptors to Cholinergic Nerve-Mediated Contractions of Murine Airway Smooth Muscle.Function (Oxford, England) · 2022Article
- Neurotransmitters responsible for purinergic motor neurotransmission and regulation of GI motility.Autonomic neuroscience : basic & clinical · 2021Article
- Mfge8 attenuates human gastric antrum smooth muscle contractions.Journal of muscle research and cell motility · 2021Article
- Excitatory cholinergic responses in mouse colon intramuscular interstitial cells of Cajal are due to enhanced CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- A novel intramuscular Interstitial Cell of Cajal is a candidate for generating pacemaker activity in the mouse internal anal sphincter.Scientific reports · 2020Article
- Phosphorylation Sites in Protein Kinases and Phosphatases Regulated by Formyl Peptide Receptor 2 Signaling.International journal of molecular sciences · 2020Review
- Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.Analytical biochemistry · 2019Article
- Spontaneous Electrical Activity and Rhythmicity in Gastrointestinal Smooth Muscles.Advances in experimental medicine and biology · 2019Review
- The functional role of protease-activated receptors on contractile responses by activation of CaAmerican journal of physiology. Gastrointestinal and liver physiology · 2018Article
- A role for focal adhesion kinase in facilitating the contractile responses of murine gastric fundus smooth muscles.The Journal of physiology · 2018Article
- The cells and conductance mediating cholinergic neurotransmission in the murine proximal stomach.The Journal of physiology · 2018Article
- Inhibitory Neural Regulation of the CaFrontiers in physiology · 2018Article
- Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes.Journal of diabetes research · 2018Article
- Physiological vs. pharmacological signalling to myosin phosphorylation in airway smooth muscle.The Journal of physiology · 2017Article
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6 authors at 2 institutions in 1 country.
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Abstract
Ca(2+) sensitization of contraction has typically been investigated by bathing muscles in solutions containing agonists. However, it is unknown whether bath-applied agonists and enteric neurotransmission activate similar Ca(2+) sensitization mechanisms. We investigated protein kinase C (PKC)-potentiated phosphatase inhibitor protein of 17 kDa (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) phosphorylation in murine gastric fundus muscles stimulated by bath-applied carbachol (CCh) or cholinergic motor neurotransmission. CCh increased MYPT1 phosphorylation at Thr696 (pT696) and Thr853 (pT853), CPI-17 at Thr38 (pT38), and myosin light chain at Ser19 (pS19). Electrical field stimulation (EFS) only increased pT38. In the presence of neostigmine, EFS increased pT38, pT853 and pS19. In fundus muscles of W/W(v) mice, EFS alone increased pT38 and pT853. Atropine blocked all contractions and all increases in pT696, pT853, pT38 and pS19. The Rho kinase (ROCK) inhibitor SAR1x blocked increases in pT853 and pT696. The PKC inhibitors Go6976 and Gf109203x or nicardipine blocked increases in pT38 and pT696. These findings suggest that cholinergic motor neurotransmission activates PKC-dependent CPI-17 phosphorylation. Bath-applied CCh recruits additional ROCK-dependent MYPT1 phosphorylation due to exposure of the agonist to a wider population of muscarinic receptors. Intramuscular interstitial cells of Cajal (ICC-IMs) and cholinesterases restrict ACh accessibility to a select population of muscarinic receptors, possibly only those expressed by ICC-IMs. These results provide the first biochemical evidence for focalized (or synaptic-like) neurotransmission, rather than diffuse 'volume' neurotransmission in a smooth muscle tissue. Furthermore, these findings demonstrate that bath application of contractile agonists to gastrointestinal smooth muscles does not mimic physiological responses to cholinergic neurotransmission.
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