ArticleThe Journal of biological chemistry2013
Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Molecular Insights into IAHSP: Influence of the R1611W Mutation on the VPS9 Domain of Alsin.ACS omega · 2025Article
- Endocytic tethers modulate unconventional GAPDH secretion.Cell surface (Amsterdam, Netherlands) · 2025Article
- Article
- Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase.G3 (Bethesda, Md.) · 2021Article
- Article
- FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity inFrontiers in microbiology · 2020Article
- Rab5 GTPases are required for optimal TORC2 function.The Journal of cell biology · 2019Article
- FolVps9, a Guanine Nucleotide Exchange Factor for FolVps21, Is Essential for Fungal Development and Pathogenicity inFrontiers in microbiology · 2019Article
- Rab5-mediated endosome formation is regulated at theCommunications biology · 2019Article
- Control of vacuole membrane homeostasis by a resident PI-3,5-kinase inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.eLife · 2017Article
- Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.FEBS letters · 2017Article
- A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection.Scientific reports · 2016Article
- Enrichment of Phosphatidylethanolamine in Viral Replication Compartments via Co-opting the Endosomal Rab5 Small GTPase by a Positive-Strand RNA Virus.PLoS biology · 2016Article
- LUCID: A Quantitative Assay of ESCRT-Mediated Cargo Sorting into Multivesicular Bodies.Traffic (Copenhagen, Denmark) · 2015Article
- Ubiquitin binding by the CUE domain promotes endosomal localization of the Rab5 GEF Vps9.Molecular biology of the cell · 2015Article
- Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes.Molecular biology of the cell · 2015Article
- The Valley-of-Death: reciprocal sign epistasis constrains adaptive trajectories in a constant, nutrient limiting environment.Genomics · 2014Article
- A Vps21 endocytic module regulates autophagy.Molecular biology of the cell · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
VPS9 domains can act as guanosine nucleotide exchange factors (GEFs) against small G proteins of the Rab5 family. Saccharomyces cerevisiae vps9Δ mutants have trafficking defects considerably less severe than multiple deletions of the three cognate Rab5 paralogs (Vps21, Ypt52, and Ypt53). Here, we show that Muk1, which also contains a VPS9 domain, acts as a second GEF against Vps21, Ypt52, and Ypt53. Muk1 is partially redundant with Vps9 in vivo, with vps9Δ muk1Δ double mutant cells displaying hypersensitivity to temperature and ionic stress, as well as profound impairments in endocytic and Golgi endosome trafficking, including defects in sorting through the multivesicular body. Cells lacking both Vps9 and Muk1 closely phenocopy double and triple knock-out strains lacking Rab5 paralogs. Microscopy and overexpression experiments demonstrate that Vps9 and Muk1 have distinct localization determinants. These experiments establish Muk1 as the second Rab5 GEF in budding yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.