ArticleMolecular therapy. Nucleic acids2013
Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus.
Article in Molecular therapy. Nucleic acids, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
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- Allele-Specific Knockdown of Mutant Huntingtin Protein via Editing at Coding Region Single Nucleotide Polymorphism Heterozygosities.Human gene therapy · 2022Article
- Application of combinatorial optimization strategies in synthetic biology.Nature communications · 2020Review
- Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing.Nature communications · 2018Article
- Opposing Roles of FANCJ and HLTF Protect Forks and Restrain Replication during Stress.Cell reports · 2018Article
- A Critical Role for the Type I Interferon Receptor in Virus-Induced Autoimmune Diabetes in Rats.Diabetes · 2017Article
- RNA activation of haploinsufficient Foxg1 gene in murine neocortex.Scientific reports · 2016Article
- DNA-binding-domain fusions enhance the targeting range and precision of Cas9.Nature methods · 2015Article
- Upregulating endogenous genes by an RNA-programmable artificial transactivator.Nucleic acids research · 2015Article
- Specific reactivation of latent HIV-1 with designer zinc-finger transcription factors targeting the HIV-1 5'-LTR promoter.Gene therapy · 2014Article
- Polyglutamine Disease Modeling: Epitope Based Screen for Homologous Recombination using CRISPR/Cas9 System.PLoS currents · 2014Article
- Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing.Cell research · 2014Article
- C-terminal in Sp1-like artificial zinc-finger proteins plays crucial roles in determining their DNA binding affinity.BMC biotechnology · 2013Article
- Mammalian Mss51 is a skeletal muscle-specific gene modulating cellular metabolism.Journal of neuromuscular diseasesArticle
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The ability to direct human telomerase reverse transcriptase (hTERT) expression through either genetic control or tunable regulatory factors would advance not only our understanding of the transcriptional regulation of this gene, but also potentially produce new strategies for addressing telomerase-associated disease. In this work, we describe the engineering of artificial zinc finger transcription factors (ZFTFs) and ZF nucleases (ZFNs) to target sequences within the hTERT promoter and exon-1. We were able to identify several active ZFTFs that demonstrate a broadly tunable response when screened by a cell-based transcriptional reporter assay. Using the same DNA-binding domains, we generated ZFNs that were screened in combinatorial pairs in cell-based extrachromosomal single-strand annealing (SSA) assays and in gene-targeting assays using stably integrated constructs. Selected ZFN pairs were tested for the ability to induce sequence changes in a Cel1 assay and we observed frequencies of genomic modification up to 18.7% at the endogenous hTERT locus. These screening strategies have pinpointed several ZFN pairs that may be useful in gene editing of the hTERT locus. Our work provides a foundation for using engineered ZF proteins (ZFPs) for modulation of the hTERT locus.Molecular Therapy - Nucleic Acids (2013) 2, e87; doi:10.1038/mtna.2013.12; published online 23 April 2013.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.