ArticleEcology and evolution2013
Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae).
Article in Ecology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- Hirudins and ornatins in five species of the genus Placobdella (Annelida: Hirudinea: Glossiphoniidae).Protoplasma · 2026Article
- Hirudins and fenestrins of the African medicinal leech Asiaticobdella fenestrata.Parasitology research · 2025Article
- Genetic Variation and Gene Expression of the Antimicrobial Peptide Macins in Asian Buffalo Leech (Biology · 2025Article
- Tracheal Hirudiniasis Induced by Ingestion of Unboiled Mountain Spring Water.The American journal of tropical medicine and hygiene · 2025Article
- Anaphylaxis to a blood feeding leech.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2025Article
- Diversity of hirudin and hirudin-like factor genes in the North-African medicinal leech, Hirudo troctina.Parasitology research · 2024Article
- Revisiting the Asian Buffalo Leech (Genes · 2023Article
- Cloning, characterization, and heterologous expression of a candidate Hirudin gene from the salivary gland transcriptome of Hirudo nipponia.Scientific reports · 2023Article
- The Origin and Evolution of Antistasin-like Proteins in Leeches (Hirudinida, Clitellata).Genome biology and evolution · 2021Article
- Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants.Scientific reports · 2020Article
- Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches.BMC genomics · 2020Article
- European Medicinal Leeches-New Roles in Modern Medicine.Biomedicines · 2020Review
- Autophagy and its role in regeneration and remodeling within invertebrate.Cell & bioscience · 2020Review
- Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the RecombinantGenes · 2019Article
- Transcriptomics and differential gene expression inEcology and evolution · 2019Article
- Are Fireworms Venomous? Evidence for the Convergent Evolution of Toxin Homologs in Three Species of Fireworms (Annelida, Amphinomidae).Genome biology and evolution · 2018Article
- An unusual cause of chest discomfort: case report of oesophageal hirudiniasis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships.Parasitology research · 2017Article
- Genome-Wide Survey of Gut Fungi (Harpellales) Reveals the First Horizontally Transferred Ubiquitin Gene from a Mosquito Host.Molecular biology and evolution · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although medicinal leeches have long been used as treatment for various ailments because of their potent anticoagulation factors, neither the full diversity of salivary components that inhibit coagulation, nor the evolutionary selection acting on them has been thoroughly investigated. Here, we constructed expressed sequence tag libraries from salivary glands of two species of medicinal hirudinoid leeches, Hirudo verbana and Aliolimnatis fenestrata, and identified anticoagulant-orthologs through BLASTx searches. The data set then was augmented by the addition of a previously constructed EST library from the macrobdelloid leech Macrobdella decora. The identified orthologs then were compared and contrasted with well-characterized anticoagulants from a variety of leeches with different feeding habits, including non-sanguivorous species. Moreover, four different statistical methods for predicting signatures of positive and negative evolutionary pressures were used for 10 rounds each to assess the level and type of selection acting on the molecules as a whole and on specific sites. In total, sequences showing putative BLASTx-orthology with five and three anticoagulant-families were recovered in the A. fenestrata and H. verbana EST libraries respectively. Selection pressure analyses predicted high levels of purifying selection across the anticoagulant diversity, although a few isolated sites showed signatures of positive selection. This study represents a first attempt at mapping the anticoagulant repertoires in a comparative fashion across several leech families.
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