ArticleNucleic acids research2013
Insulator protein Su(Hw) recruits SAGA and Brahma complexes and constitutes part of Origin Recognition Complex-binding sites in the Drosophila genome.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 69 citations in OpenAlex.
- Novel principles of molecular genetic mapping of the interphase genome of Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- SRP/dGATAb modulates the ecdysone response bidirectionally via locus-specific regulatory landscape.Epigenetics & chromatin · 2025Article
- Vostok: A looping factor for the organization of the regulatory genome in the Drosophila brain.Molecular cell · 2025Article
- Su(Hw) interacts with Combgap to establish long-range chromatin contacts.Epigenetics & chromatin · 2024Article
- Coregulators Reside withinInternational journal of molecular sciences · 2023Article
- RNA Polymerase II "Pause" Prepares Promoters for Upcoming Transcription duringInternational journal of molecular sciences · 2022Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- Su(Hw) primes 66D and 7F Drosophila chorion genes loci for amplification through chromatin decondensation.Scientific reports · 2021Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- Multi-Scale Organization of theGenes · 2021Review
- The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes.Scientific reports · 2021Article
- Mutations in the insulator protein Suppressor of Hairy wing induce genome instability.Chromosoma · 2020Article
- Proximity-dependent biotin labelling reveals CP190 as an EcR/Usp molecular partner.Scientific reports · 2020Article
- HIPP1 stabilizes the interaction between CP190 and Su(Hw) in the Drosophila insulator complex.Scientific reports · 2019Article
- The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression.Scientific reports · 2019Article
- Spermiogenesis and Male Fertility Require the Function of Suppressor of Hairy-Wing in Somatic Cyst Cells ofGenetics · 2018Article
- Identification of the ORC Complex Subunits That Can Interact with the ENY2 Protein of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2018Article
- Study of the Ability of the gypsy Insulator to Stabilize Amplification of the chorion Replication Origin of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2018Article
- Insulator Protein Su(Hw) Is Indispensable for Amplification of Part of Drosophila Amplicons in Follicular Cells (DAFCs) during Early Oogenesis.Doklady. Biochemistry and biophysics · 2018Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
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9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Despite increasing data on the properties of replication origins, molecular mechanisms underlying origin recognition complex (ORC) positioning in the genome are still poorly understood. The Su(Hw) protein accounts for the activity of best-studied Drosophila insulators. Here, we show that Su(Hw) recruits the histone acetyltransferase complex SAGA and chromatin remodeler Brahma to Su(Hw)-dependent insulators, which gives rise to regions with low nucleosome density and creates conditions for ORC binding. Depletion in Su(Hw) leads to a dramatic drop in the levels of SAGA, Brahma and ORC subunits and a significant increase in nucleosome density on Su(Hw)-dependent insulators, whereas artificial Su(Hw) recruitment itself is sufficient for subsequent SAGA, Brahma and ORC binding. In contrast to the majority of replication origins that associate with promoters of active genes, Su(Hw)-binding sites constitute a small proportion (6%) of ORC-binding sites that are localized preferentially in transcriptionally inactive chromatin regions termed BLACK and BLUE chromatin. We suggest that the key determinants of ORC positioning in the genome are DNA-binding proteins that constitute different DNA regulatory elements, including insulators, promoters and enhancers. Su(Hw) is the first example of such a protein.
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