ArticleThe Journal of biological chemistry2013
Dynamic structure of the translocon SecYEG in membrane: direct single molecule observations.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Automated registration and clustering for enhanced localization atomic force microscopy of flexible membrane proteins.PLoS computational biology · 2025Article
- Article
- Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity.International journal of molecular sciences · 2022Review
- TheeLife · 2022Article
- Controllable membrane remodeling by a modified fragment of the apoptotic protein Bax.Faraday discussions · 2021Article
- Atomic Force Microscopy to Elicit Conformational Transitions of Ferredoxin-Dependent Flavin Thioredoxin Reductases.Antioxidants (Basel, Switzerland) · 2021Article
- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Interaction of the motor protein SecA and the bacterial protein translocation channel SecYEG in the absence of ATP.Nanoscale advances · 2020Article
- Protein Translocation Activity in Surface-Supported Lipid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Direct visualization of theScience advances · 2019Article
- AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density.Methods and protocols · 2019Article
- Quaternary structure of the small amino acid transporter OprG fromThe Journal of biological chemistry · 2018Article
- Single-molecule observation of nucleotide induced conformational changes in basal SecA-ATP hydrolysis.Science advances · 2018Article
- The conformation and dynamics of P-glycoprotein in a lipid bilayer investigated by atomic force microscopy.Biochemical pharmacology · 2018Article
- The Hessian Blob Algorithm: Precise Particle Detection in Atomic Force Microscopy Imagery.Scientific reports · 2018Article
- The Sec System: Protein Export inEcoSal Plus · 2017Review
- Protein export through the bacterial Sec pathway.Nature reviews. Microbiology · 2017Review
- Lipids Activate SecA for High Affinity Binding to the SecYEG Complex.The Journal of biological chemistry · 2016Article
- A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform.The Plant cell · 2016Article
- Glass is a Viable Substrate for Precision Force Microscopy of Membrane Proteins.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Purified SecYEG was reconstituted into liposomes and studied in near-native conditions using atomic force microscopy. These SecYEG proteoliposomes were active in translocation assays. Changes in the structure of SecYEG as a function of time were directly visualized. The dynamics observed were significant in magnitude (∼1-10 Å) and were attributed to the two large loops of SecY linking transmembrane helices 6-7 and 8-9. In addition, we identified a distribution between monomers and dimers of SecYEG as well as a smaller population of higher order oligomers. This work provides a new vista of the flexible and dynamic structure of SecYEG, an intricate and vital membrane protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.