ArticleThe Journal of biological chemistry2013
Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 141 citations in OpenAlex.
- Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging.Bioanalysis · 2025Review
- Depth Correction of TOF-SIMS Depth Profiling Images Using the Total Ion Count Images.Biomolecules · 2025Article
- Serotonin Signaling through Lipid Membranes.ACS chemical neuroscience · 2024Review
- Subcellular protein turnover in human neural progenitor cells revealed by correlative electron microscopy and nanoscale secondary ion mass spectrometry imaging.Chemical science · 2024Article
- Mass Spectrometry Imaging of Cholesterol and Oxysterols.Advances in experimental medicine and biology · 2024Article
- Molecular mechanisms of spontaneous curvature and softening in complex lipid bilayer mixtures.Biophysical journal · 2022Article
- Lipid-driven CFTR clustering is impaired in cystic fibrosis and restored by corrector drugs.Journal of cell science · 2022Article
- Plasma membrane effects of sphingolipid-synthesis inhibition by myriocin in CHO cells: a biophysical and lipidomic study.Scientific reports · 2022Article
- Advancing cell instructive biomaterials through increased understanding of cell receptor spacing and material surface functionalization.Regenerative engineering and translational medicine · 2021Article
- Article
- Depth correction of 3D NanoSIMS images using secondary electron pixel intensities.Biointerphases · 2021Article
- Cholesterol-dependent plasma membrane order (LScientific reports · 2021Article
- CHO/LY-B cell growth under limiting sphingolipid supply: Correlation between lipid composition and biophysical properties of sphingolipid-restricted cell membranes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene.Nature methods · 2021Article
- Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Elastic deformations mediate interaction of the raft boundary with membrane inclusions leading to their effective lateral sorting.Scientific reports · 2020Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- Folding and Misfolding of Human Membrane Proteins in Health and Disease: From Single Molecules to Cellular Proteostasis.Chemical reviews · 2019Article
- Influenza Hemagglutinin Modulates Phosphatidylinositol 4,5-Bisphosphate Membrane Clustering.Biophysical journal · 2019Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the plasma membranes of intact fibroblast cells. Although acute cholesterol depletion reduced sphingolipid domain abundance, cholesterol was evenly distributed throughout the plasma membrane and was not enriched within the sphingolipid domains. Thus, we rule out favorable cholesterol-sphingolipid interactions as dictating plasma membrane organization in fibroblast cells. Because the sphingolipid domains are disrupted by drugs that depolymerize the cells actin cytoskeleton, cholesterol must instead affect the sphingolipid organization via an indirect mechanism that involves the cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.