ArticleJournal of the American Society for Mass Spectrometry2013
Multipurpose dissociation cell for enhanced ETD of intact protein species.
Article in Journal of the American Society for Mass Spectrometry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- A nature-inspired ion trap for parallel manipulation of ions on a massive scale.Science advances · 2026Article
- Implementation of Infrared-Activated Negative Electron Transfer Dissociation (IR-NETD) Using Xenon on a Quadrupole-Orbitrap-Quadrupole Linear Ion Trap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2026Article
- Instrumentation at the Leading Edge of Proteomics.Analytical chemistry · 2024Review
- Increased Single-Spectrum Top-Down Protein Sequence Coverage in Trapping Mass Spectrometers with Chimeric Ion Loading.Analytical chemistry · 2020Article
- Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure.Mass spectrometry reviews · 2018Review
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
- Negative Electron Transfer Dissociation Sequencing of Increasingly Sulfated Glycosaminoglycan Oligosaccharides on an Orbitrap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2017Article
- Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis.Journal of the American Society for Mass Spectrometry · 2017Article
- Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.Journal of proteome research · 2017Article
- Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2017Article
- Implementation of Activated Ion Electron Transfer Dissociation on a Quadrupole-Orbitrap-Linear Ion Trap Hybrid Mass Spectrometer.Analytical chemistry · 2017Article
- Top-down analysis of immunoglobulin G isotypes 1 and 2 with electron transfer dissociation on a high-field Orbitrap mass spectrometer.Journal of proteomics · 2017Article
- Full-Featured Search Algorithm for Negative Electron-Transfer Dissociation.Journal of proteome research · 2016Article
- Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2016Article
- A calibration routine for efficient ETD in large-scale proteomics.Journal of the American Society for Mass Spectrometry · 2015Article
- The Negative Mode Proteome with Activated Ion Negative Electron Transfer Dissociation (AI-NETD).Molecular & cellular proteomics : MCP · 2015Article
- Comparison of Ultraviolet Photodissociation and Collision Induced Dissociation of Adrenocorticotropic Hormone Peptides.Journal of the American Society for Mass Spectrometry · 2015Article
- Activated Ion Electron Transfer Dissociation for Improved Fragmentation of Intact Proteins.Analytical chemistry · 2015Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
We describe and characterize an improved implementation of ETD on a modified hybrid linear ion trap-Orbitrap instrument. Instead of performing ETD in the mass-analyzing quadrupole linear ion trap (A-QLT), the instrument collision cell was modified to enable ETD. We partitioned the collision cell into a multi-section rf ion storage and transfer device to enable injection and simultaneous separate storage of precursor and reagent ions. Application of a secondary (axial) confinement voltage to the cell end lens electrodes enables charge-sign independent trapping for ion-ion reactions. The approximately 2-fold higher quadrupole field frequency of this cell relative to that of the A-QLT enables higher reagent ion densities and correspondingly faster ETD reactions, and, with the collision cell's longer axial dimensions, larger populations of precursor ions may be reacted. The higher ion capacity of the collision cell permits the accumulation and reaction of multiple full loads of precursor ions from the A-QLT followed by FT Orbitrap m/z analysis of the ETD product ions. This extends the intra-scan dynamic range by increasing the maximum number of product ions in a single MS/MS event. For analyses of large peptide/small protein precursor cations, this reduces or eliminates the need for spectral averaging to achieve acceptable ETD product ion signal-to-noise levels. Using larger ion populations, we demonstrate improvements in protein sequence coverage and aggregate protein identifications in LC-MS/MS analysis of intact protein species as compared to the standard ETD implementation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.