ArticleJournal of visualized experiments : JoVE2013
Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Compression injury regulates astrocyte morphology, metabolic function, and extracellular matrix modification in a 3D hydrogel.bioRxiv : the preprint server for biology · 2025Article
- Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius Neurons.International journal of molecular sciences · 2023Article
- Delayed cortical development in mice with a neural specific deletion of β1 integrin.Frontiers in neuroscience · 2023Article
- Maternal Ethanol Exposure Acutely Elevates Src Family Kinase Activity in the Fetal Cortex.Molecular neurobiology · 2021Article
- Ethanol Exposure Transiently Elevates but Persistently Inhibits Tyrosine Kinase Activity and Impairs the Growth of the Nascent Apical Dendrite.Molecular neurobiology · 2019Article
- Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons.Journal of visualized experiments : JoVE · 2018Article
- Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation.Development (Cambridge, England) · 2017Article
- Genetic and Molecular Approaches to Study Neuronal Migration in the Developing Cerebral Cortex.Brain sciences · 2017Review
- Reelin Prevents Apical Neurite Retraction during Terminal Translocation and Dendrite Initiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Ex utero electroporation and organotypic slice culture of mouse hippocampal tissue.Journal of visualized experiments : JoVE · 2015Article
- Mouse hindbrain ex vivo culture to study facial branchiomotor neuron migration.Journal of visualized experiments : JoVE · 2014Article
- Analysis of preplate splitting and early cortical development illuminates the biology of neurological disease.Frontiers in pediatrics · 2014Review
- Reelin Counteracts Chondroitin Sulfate Proteoglycan-Mediated Cortical Dendrite Growth Inhibition.eNeuroArticle
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4 authors.
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Abstract
Cortical development involves complex interactions between neurons and non-neuronal elements including precursor cells, blood vessels, meninges and associated extracellular matrix. Because they provide a suitable organotypic environment, cortical slice explants are often used to investigate those interactions that control neuronal differentiation and development. Although beneficial, the slice explant model can suffer from drawbacks including aberrant cellular lamination and migration. Here we report a whole cerebral hemisphere explant system for studies of early cortical development that is easier to prepare than cortical slices and shows consistent organotypic migration and lamination. In this model system, early lamination and migration patterns proceed normally for a period of two days in vitro, including the period of preplate splitting, during which prospective cortical layer six forms. We then developed an ex utero electroporation (EUEP) approach that achieves -80% success in targeting GFP expression to neurons developing in the dorsal medial cortex. The whole hemisphere explant model makes early cortical development accessible for electroporation, pharmacological intervention and live imaging approaches. This method avoids the survival surgery required of in utero electroporation (IUEP) approaches while improving both transfection and areal targeting consistency. This method will facilitate experimental studies of neuronal proliferation, migration and differentiation.
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