ArticleJournal of industrial microbiology & biotechnology2013
Genome-scale modeling enables metabolic engineering of Saccharomyces cerevisiae for succinic acid production.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Succinate and lactate produced as conserved biomarkers through chronic and transient substrate-level phosphorylation: from microorganisms to cancer.Journal of bioenergetics and biomembranes · 2026Review
- Succinic Acid Production by Engineered Mannheimia succiniciproducens and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis.Biotechnology and bioengineering · 2025Article
- Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.FEMS yeast research · 2025Review
- High-level succinic acid production by overexpressing a magnesium transporter inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Response mechanisms of different Saccharomyces cerevisiae strains to succinic acid.BMC microbiology · 2024Article
- Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation.Applied microbiology and biotechnology · 2024Article
- Co-Cultivated Enzyme Constraint Metabolic Network Model for Rational Guidance in Constructing Synthetic Consortia to Achieve Optimal Pathway Allocation Prediction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A genome-scale metabolic model of the effect of dissolved oxygen on 1,3-propanediol fermentation by Klebsiella pneumoniae.Bioprocess and biosystems engineering · 2023Article
- A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli.Biotechnology and bioengineering · 2022Article
- Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts.Microbial cell factories · 2021Article
- Model-guided development of an evolutionarily stable yeast chassis.Molecular systems biology · 2021Article
- The Role of Yeasts and Lactic Acid Bacteria on the Metabolism of Organic Acids during Winemaking.Foods (Basel, Switzerland) · 2020Review
- Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase.Nature communications · 2020Article
- QTL mapping of modelled metabolic fluxes reveals gene variants impacting yeast central carbon metabolism.Scientific reports · 2020Article
- Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose.Microbial cell factories · 2019Article
- Evaluation of pyruvate decarboxylase-negativeEngineering in life sciences · 2019Article
- Metabolic fluxes-oriented control of bioreactors: a novel approach to tune micro-aeration and substrate feeding in fermentations.Microbial cell factories · 2019Article
- OptRAM: In-silico strain design via integrative regulatory-metabolic network modeling.PLoS computational biology · 2019Article
- Genome-Scale Metabolic Modeling from Yeast to Human Cell Models of Complex Diseases: Latest Advances and Challenges.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Genome-scale modeling of yeast: chronology, applications and critical perspectives.FEMS yeast research · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we describe the application of a genome-scale metabolic model and flux balance analysis for the prediction of succinic acid overproduction strategies in Saccharomyces cerevisiae. The top three single gene deletion strategies, Δmdh1, Δoac1, and Δdic1, were tested using knock-out strains cultivated anaerobically on glucose, coupled with physiological and DNA microarray characterization. While Δmdh1 and Δoac1 strains failed to produce succinate, Δdic1 produced 0.02 C-mol/C-mol glucose, in close agreement with model predictions (0.03 C-mol/C-mol glucose). Transcriptional profiling suggests that succinate formation is coupled to mitochondrial redox balancing, and more specifically, reductive TCA cycle activity. While far from industrial titers, this proof-of-concept suggests that in silico predictions coupled with experimental validation can be used to identify novel and non-intuitive metabolic engineering strategies.
Indexed as
Identifiers
23608777What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.