ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2013
Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Measuring the Severity of Respiratory Illness in the First 2 Years of Life in Preterm and Term Infants.The Journal of pediatrics · 2019Trial
- Influenza Among Young Children in Bangladesh: Clinical Characteristics and Outcomes From a Randomized Clinical Trial.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Trial
- Maternal vitamin D supplementation during pregnancy and lactation to prevent acute respiratory infections in infancy in Dhaka, Bangladesh (MDARI trial): protocol for a prospective cohort study nested within a randomized controlled trial.BMC pregnancy and childbirth · 2016Trial
- Incidence, viral etiology, and risk factors of wheezing in children under two years of age: findings from a low-income urban community in Dhaka, Bangladesh.BMC pulmonary medicine · 2026Article
- Incidence and Viral Etiology of Acute Respiratory Infections and Pneumonia among Children Under Two Years: A Birth Cohort Study in Dhaka, Bangladesh.The American journal of tropical medicine and hygiene · 2026Article
- Acute febrile illness surveillance using TaqMan Array Cards in two urban health facilities, Monrovia, Liberia, December 2018-March 2020.PLoS neglected tropical diseases · 2026Article
- Performance evaluation of TaqMan Array Card real-time PCR for multi-pathogen detection in acute undifferentiated febrile illness.BMC infectious diseases · 2026Article
- Article
- Distinct Evolutionary Signatures of Human Parainfluenza Viruses 2 and 4 Reveal Host Antagonism Divergence and Phylogenetic Discordance.Molecular biology and evolution · 2025Article
- Estimated Vaccine Effectiveness for Pediatric Patients With Severe Influenza, 2015-2020.JAMA network open · 2024Article
- Respiratory Syncytial Virus-Associated Deaths among Children under Five before and during the COVID-19 Pandemic in Bangladesh.Viruses · 2024Article
- Enhanced pathogen identification among patients with clinically suspected meningitis.Southern African journal of infectious diseases · 2024Article
- Simultaneous detection and quantification of multiple pathogen targets in wastewater.medRxiv : the preprint server for health sciences · 2023Article
- Article
- Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness.Diagnostics (Basel, Switzerland) · 2023Article
- The rapid detection of respiratory pathogens in critically ill children.Critical care (London, England) · 2023Observational
- Pre COVID-19 molecular epidemiology of respiratory syncytial virus (RSV) among children in Bangladesh.Heliyon · 2022Article
- Infant deaths from respiratory syncytial virus in Lusaka, Zambia from the ZPRIME study: a 3-year, systematic, post-mortem surveillance project.The Lancet. Global health · 2022Article
- Side-by-side comparison of parent vs. technician-collected respiratory swabs in low-income, multilingual, urban communities in the United States.BMC public health · 2022Article
- Severe Acute Respiratory Syndrome Coronavirus 2 and Respiratory Virus Sentinel Surveillance, California, USA, May 10, 2020-June 12, 2021.Emerging infectious diseases · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMultipathogen reverse transcription real-time PCR (RT-qPCR) platforms have proven useful in surveillance for acute respiratory illness (ARI) and study of respiratory outbreaks of unknown etiology. The TaqMan(®) Array Card (TAC, Life Technologies™), can simultaneously test 7 clinical specimens for up to 21 individual pathogens (depending on arrangement of controls and use of duplicate wells) by arrayed singleplex RT-qPCR on a single assay card, using minimal amounts of clinical specimens. A previous study described the development of TAC for the detection of respiratory viral and bacterial pathogens; the assay was evaluated against well-characterized analytical materials and a limited collection of human clinical specimens.
objectivesWe wished to compare TAC assay performance against standard individual RT-qPCR assays for respiratory viral detection, focusing on 10 viruses (adenovirus, human metapneumovirus, human parainfluenza viruses 1-4, influenza viruses A and B, respiratory syncytial virus, and rhinovirus) from a larger collection of human specimens. STUDY
designWe used specimens from 942 children with ARI enrolled systematically in a population-based, ARI surveillance study (New Vaccine Surveillance Network, NVSN).
resultsCompared with standard individual RT-qPCR assays, the sensitivity of TAC for the targeted viruses ranged from 54% to 95% (54%, 56%, and 75% for adenovirus, human parainfluenza viruses-1 and -2, respectively, and 82%-95% for the other viruses). Assay specificity was 99%, and coefficients of variation for virus controls ranged from 1.5% to 4.5%.
conclusionThe TAC assay should prove useful for multipathogen studies and rapid outbreak response.
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